Enzyme-triggered formation of enzyme-tyramine concatamers on nanogold-functionalized dendrimer for impedimetric detection of Hg(II) with sensitivity enhancement》.pdf

Biosensors and Bioelectronics 75 (2016) 108–115 Contents lists available at ScienceDirect Biosensors and Bioelectronics journal homepage: /locate/bios Enzyme-triggered formation of enzyme-tyramine concatamers on nanogold-functionalized dendrimer for impedimetric detection of Hg(II) with sensitivity enhancement Zhenli Qiu a, Dianyong Tang a,b,n, Jian Shu a, Guonan Chen a, Dianping Tang a,n a Key Laboratory of Analysis and Detection for Food Safety (MOE Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China b Chongqing Key Laboratory of Environmental Materials Remediation Technologies, Chongqing University of Arts and Sciences, Chongqing 402160, PR China a r t i c l e i n f o a b s t r a c t Article history: A new impedimetric sensing strategy based on enzyme-triggered formation of enzyme-tyramine con- Received 30 June 2015 catamers on the nanogold-functionalized poly(amidoamine) (PAMAM) dendrimer was designed for Received in revised form sensitive detection of mercury(II) (Hg2 þ ) ion, coupling with enzymatic biocatalytic precipitation towards 8 August 2015 4-choloro-1-naphthol (4-CN) on thymine (T)-rich single-stranded DNA -modified electrode. Initially, 1 Accepted 14 August 2015 nanogold–decorated PAMAM dend