Esophageal Injury with External Esophageal Perfusion1》.pdf

Journal of Surgical Research 129, 107–113 (2005) doi:10.1016/j.jss.2005.04.018 Esophageal Injury with External Esophageal Perfusion1 Yan Li, M.D., Ph.D.,* John M. Wo, M.D.,† Ruifeng R. Su, B.S.,* Mukunda B. Ray, M.D., Ph.D.,‡ Whitney Jones, M.D.,† and Robert C.G. Martin, M.D.*,2 *Division of Surgical Oncology; †Division of Gastroenterology/Hepatology; ‡Department of Anatomical Pathology, University of Louisville School of Medicine, Louisville, Kentucky Submitted for publication February 2, 2005 changes seen in severe reflux esophagitis. The cellular Background. External esophageal perfusion (EEP) changes in apoptosis, proliferation and anti-oxidant with the idea that esophageal perfusion can be con- defense make this model unique for reflux esophagitis trolled with a single ingredient at a constant rate and studies. Further studies are needed to induce Barrett’s concentration, might be used to dissect the injurious esophagus and esophageal adenocarcinoma. © 2005 role of gastro-duodenal secretions for the progression Elsevier Inc. All rights reserved. from esophagitis to Barrett’s esophagus (BE) and Key Words: gastroesophageal reflux; esophagitis; an- esophageal adenocarcinoma (EAC). This study is to imal model; oxidative stress. evaluate the EEP rat model for esophagitis induced by using a mirco-osmotic pump with bile perfusion. INTRODUCTION Methods. Eighteen adult rats underwent the EEP procedure. Bile (0.5% bovine bile, pH 7.4) was used as perfusion agent and three t