Establishment and evaluation of 2 monoclonal antibodies against oxidized apolipoprotein A-I (apoA-I) and its application to determine blood oxidized apoA-I levels》.pdf

Clinica Chimica Acta 378 (2007) 105 – 111 /locate/clinchim Establishment and evaluation of 2 monoclonal antibodies against oxidized apolipoprotein A-I (apoA-I) and its application to determine blood oxidized apoA-I levels Masashi Ueda ⁎, Yasuyuki Hayase, Shinichi Mashiba Ikagaku Co. Ltd., Furukawacho, 328 Hazukashi Fushimi-ku, Kyoto 612-8486, Japan Received 5 July 2006; received in revised form 31 October 2006; accepted 1 November 2006 Available online 10 November 2006 Abstract Background: Apolipoprotein A-I (apoA-I) is the major lipoprotein component of high-density lipoprotein(HDL), and plays an important role in reverse cholesterol transport. Its function is known to be influenced by oxidation. Methods: Using H O - or chloramine T-oxidized apoA-I as antigen, we prepared 2 kinds of monoclonal antibodies, and established an ELISA 2 2 system for the measurement of oxidized apoA-I. Results: The 2 monoclonal antibodies obtained, 7D3 and 98A7, exhibited different reactivity characteristics. The serum level of oxidized apoA-I was higher in patients with either inflammatory disease or diabetes than in healthy individuals, and suggested a diversity of oxidized apoA-I. Conclusion: The 2 monoclonal antibodies are useful for the determination of oxidized apoA-I and study of diverse oxidized HDLs. © 2006 Elsevier B.V. All rights reserved. Keywords: Apolipoprotein A-I; Oxidized apolipoprotein A-I; Monoclonal antibody; Oxidative stress; ELISA 1. Introduction [