Generation of Gene-Modified Cynomolgus Monkey via Cas9RNA-Mediated Gene Targeting in One-Cell Embryos》.pdf

Resource Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos Yuyu Niu,1,5,7 Bin Shen,2,7 Yiqiang Cui,3,7 Yongchang Chen,1,5,7 Jianying Wang,2 Lei Wang,3 Yu Kang,1,5 Xiaoyang Zhao,4 Wei Si,1,5 Wei Li,4 Andy Peng Xiang,6 Jiankui Zhou,2 Xuejiang Guo,3 Ye Bi,3 Chenyang Si,1,5 Bian Hu,2 Guoying Dong,3 Hong Wang,1,5 Zuomin Zhou,3 Tianqing Li,1,5 Tao Tan,1,5 Xiuqiong Pu,1,5 Fang Wang,1,5 Shaohui Ji,1,5 Qi Zhou,4 Xingxu Huang,2,* Weizhi Ji,1,5,* and Jiahao Sha3,* 1Yunnan Key Laboratory of Primate Biomedical Research, Kunming 650500, China 2MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing 210061, China 3State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China 4State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China 5Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, China 6Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Sun Yat-Sen University, Guangzhou 510080, China 7These authors contributed equally to this work *Correspondence: shajh@ (J.S.), wji@ (W.J.), xingxuhuang@ (X.H.) /10.1016/j.cell.2014.01.027 SUMMARY Sasaki et al., 2009; Yang et al., 2008), precise genomic targeting in monkeys is the most desired for generating human disease Monkeys serve as important model species for models and has not been achieved so far (Chan, 2013; S