Genetic variants in ABCA1 promoter affect transcription activity and plasma HDL level in pigs》.pdf

Gene 555 (2015) 414–420 Contents lists available at ScienceDirect Gene journal homepage: www. /locate/gene Genetic variants in ABCA1 promoter affect transcription activity and plasma HDL level in pigs Xiao-yong Dang a, Wei-wei Chu a, Heng-chuan Shi b, Shi-gang Yu a, Hai-yin Han a, Shu-hua Gu a, Jie Chen a,⁎ a College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China b Laboratory Department, Jiangsu Province Offi cial Hospital, Nanjing 210024, PR China a r t i c l e i n f o a b s t r a c t Article history: Excess accumulation of cholesterol in plasma may result in coronary artery disease. Numerous studies have dem- Received 24 September 2014 onstrated that ATP-binding cassette protein A1 (ABCA1 ) mediates the efflux of cholesterol and phospholipids to Received in revised form 11 November 2014 apolipoproteins, a process necessary for plasma high density lipoprotein (HDL) formation. Higher plasma levels Accepted 19 November 2014 of HDL are associated with lower risk for cardiovascular disease. Studies of human disease and animal models had Available online 21 November 2014 shown that an increased hepatic ABCA1 activity relates to an enhanced plasma HDL level. In this study, we hy- Keywords: pothesized that functional mutations in the ABCA1 promoter in pigs may affect gene transcription activity, and ABCA1 consequently the HDL l