Genomic cloning and promoter functional analysis of myostatin-2 in shi drum, Umbrina cirrosa Conservation of muscle-specific promoter activity》.pdf

Comparative Biochemistry and Physiology, Part B 164 (2013) 99–110 Contents lists available at SciVerse ScienceDirect Comparative Biochemistry and Physiology, Part B journal homepage: /locate/cbpb Genomic cloning and promoter functional analysis of myostatin-2 in shi drum, Umbrina cirrosa: Conservation of muscle-specific promoter activity Elisabeth Nadjar-Boger a, Lisa Maccatrozzo b, Giuseppe Radaelli b, Bruria Funkenstein a,⁎ a Department of Marine Biology Biotechnology, National Institute of Oceanography, Israel Oceanographic and Limnological Research, Tel-Shikmona, P.O.B 8030, Haifa 31080, Israel b Department of Experimental Veterinary Sciences, Faculty of Veterinary Medicine, University of Padua, Italy a r t i c l e i n f o a b s t r a c t Article history: Myostatin (MSTN ) is a member of the transforming growth factor-ß superfamily, known as a negative regulator of Received 1 October 2012 skeletal muscle development and growth in mammals. In contrast to mammals, fish possess at least two paralogs Received in revised form 13 November 2012 of MSTN: MSTN-1 and MSTN-2. Here we describe the cloning and sequence analysis of spliced and precursor Accepted 14 November 2012 (unspliced) transcripts as well as the 5′ flanking region of MSTN-2 from the marine fish Umbrina cirrosa Available online 21 November 2012 (ucMSTN-2). In silico analysis revealed numerous putative cis regulatory elements including several E-boxes known as binding site