Myeloperoxidase-mediated oxidation targets serum apolipoprotein A-I in diabetic patients and represents a potential mechanism leading to impaired anti-apoptotic activity of high density lipoprotein》.pdf

Clinica Chimica Acta 441 (2015) 163–170 Contents lists available at ScienceDirect Clinica Chimica Acta journal homepage: www. else vi er. com/ locate /clinchim Myeloperoxidase-mediated oxidation targets serum apolipoprotein A-I in diabetic patients and represents a potential mechanism leading to impaired anti-apoptotic activity of high density lipoprotein Naihao Lu a,⁎, Shiliang Xie b, Jiayu Li b, Rong Tian b, Yi-Yuan Peng a,b,⁎⁎ a Key Laboratory of Functional Small Organic Molecule, Ministry of Education and College of Life Science, Jiangxi Normal University, 99 Ziyang Road, Nanchang, Jiangxi 330022, China b Key Laboratory of Green Chemistry, Jiangxi Province and College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang, Jiangxi, 330022, China a r t i c l e i n f o a b s t r a c t Article history: Background: It is demonstrated that levels of protein-bound chlorotyrosine, nitrotyrosine and myeloperoxidase Received 9 September 2014 (MPO), a protein that catalyzes generation of chlorinating and nitrating oxidants, serve as independent predictors Received in revised form 7 December 2014 of cardiovascular disease. Accepted 10 December 2014 Methods: Immunoprecipitation and Western blot were used to analyze protein concentration, nitration and Available online 17 December 2014 chlorination. LC-MS/MS was used to identify nitrated and chlorinated sites of Tyr from immunoprecipitated serum proteins. Keywords: Diabetes