p32, a novel binding partner of Mcl-1, positively regulates mitochondrial Ca2+ uptake and apoptosis》.pdf

Biochemical and Biophysical Research Communications 451 (2014) 322–328 Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.else /loc ate/ybbrc p32, a novel binding partner of Mcl-1, positively regulates mitochondrial Ca2+ uptake and apoptosis Kang Xiao a, Yinyin Wang b, Zhijie Chang b, Yuanzhi Lao c,⇑, Donald C. Chang a,⇑ a Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China b School of Medicine, Tsinghua University, Beijing, China c School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China a r t i c l e i n f o a b s t r a c t Article history: Mcl-1 is a major anti-apoptotic Bcl-2 family protein. It is well known that Mcl-1 can interact with certain Received 23 July 2014 pro-apoptotic Bcl-2 family proteins in normal cells to neutralize their pro-apoptotic functions, thus pre- Available online 1 August 2014 vent apoptosis. In addition, it was recently found that Mcl-1 can also inhibit mitochondrial calcium uptake. The detailed mechanism, however, is still not clear. Based on Yeast Two-Hybrid screening and Keywords: co-immunoprecipitation, we identified a mitochondrial protein p32 (C1qbp) as a novel binding partner Apoptosis of Mcl-1. We found that p32 had a number of interesting properties: (1) p32 can positively regulate Mcl-1 UV-induced apoptosis