兽医血液学和细胞学.pdf

Hematology and Cytology: More than pretty Colors Sharon M. Dial, DVM, PhD, DACVP Arizona Veterinary Diagnostic Laboratory University of Arizona Quality from the Beginning Hematology and Clinical Chemistry data is only as good as the sample submitted Pre-analysis factors can effect all data Is the sample fasting? how was the blood drawn? how was the sample handled post-draw? Sample Handling Blood collection: Label, label, label slides and blood tubes Use frosted end slides - label in pencil add paper label after staining If submitting to lab - no paper labels Sample Handling Use largest bore needle possible decrease time for collection decreases lysis of fragile RBCs Vacutainer versus needle and syringe? whatever gives you the best sample Consider use of a butterfly catheter with Vacutainer connection. Collecting the Blood Sample Principles of good venipuncture #21 gauge or larger facilitates rapid flow, minimizes hemolysis. move plunger within unit to ensure syringe patency. enter the vein keeping bevel side of needle up. Avoid repositioning or excessive suction. 1 Collecting the Blood Sample Immediately transfer blood to an EDTA collection tube. Method 1 Remove needle and tube stopper. Gently dispense the blood into the tube. Method 2 Leave needle on and push it through the stopper allow the vacuu