杂交籼稻骨干保持系岗46B稻谷粒形及垩白的QTL分析.doc

杂交籼稻骨干保持系岗４６B稻谷粒形及垩白的QTL分析 高方远１,＃　邱玲１,２,＃　陆贤军１　任鄄胜１　吴贤婷１　任光俊１　曾礼华２, (１四川省农业科学院作物研究所,成都６１００６６;２四川师范大学,成都６１０１１０;＃共同第一作者;通讯联系人,EＧmail:gfy２４６＠ １６３．com;zenglihua６６＠hotmail．com) QTLAnalysisonGrainShapeandChalkinessofanEliteMaintainerLineGang ４６BinHybridRice(OryzasativaL．) GAOFangＧyuan１,＃,QIULing１,２,＃,LUXianＧjun１,RENJuanＧsheng１,WUXianＧting１,RENGuangＧjun１, ZENGLiＧhua２, (１CropResearchInstitute,SichuanAcademyofAgriculturalSciences,Chengdu６１００６６,China;２SichuanNormalUniversity, Chengdu６１０１１０,China;＃Theseauthorscontributedequallytothispaper;Correspondingauthor,EＧmail:gfy２４６＠１６３．com, zenglihua６６＠hotmail．com) GAOFangyuan,QIULing,LUXianjun,etal．QTLanalysisongrainshapeandchalkinessofanelitemaintainerline Gang４６Binhybridrice(OryzasativaL．)．ChinJRiceSci,２０１４,２８(３):２３５Ｇ２４２． Abstract:Toidentifythegeneticlocithatcontrolgrainlength,width,lengthＧwidthratio,thickness,１００Ｇgrainweight andthepercentageofgrainswithchalkiness(GL,GW,LWR,GT,HGW,PGWC),weconstructedanF２population bycrossinganeliteindicamaintainerlineGang４６B(G４６B)withK１０７５,anintrogressionlineofG４６Bderivedfrom multiＧbackcrosswithanUSricevarietyLemont,withlowpercentageofchalkinessandnarrowgrain．Allsix investigatedtraitsfollowedacontinuousphenotypicdistribution．Significantlypositiveornegativecorrelationswere observedbetweenallsixtraits,excepttheGLandGW．Ageneticmapwasconstructedwith３０simplesequencerepeat (SSR)markersandatotalof１４QTLsforgrainshapeandchalkinessweredetectedbycompositeintervalmapping (CIM)．Amongwhich,twowereforGL(qGL２andqGL５),twoforGW(qGW５andqGW８),fiveforLWR (qLWR２,qLWR５,qLWR６,qLWR８andqLWR１２),oneforGT(qGT５),oneforHGW(qHGW５),andthreefor PGWC(qPGWC５,qPGWC６andqPGWC８)．TheG４６BallelescontributedtoincreasingGW,GT,HGWandPGWC, whiledecreasingGLandLWRatalldetectedloci．ThemajorQTLscontrollingGW,GT,GW,PGWCandLWRwere locatedinthesameregionRM１７９９０ＧRM１８００４ＧRM１８０６８onchromosome５,whichwascorrespondenttotheresults identifiedinextremephenotypeplantsusinglinkageandlinkagedisequilibrium． Keywords:rice;grainshape;chalkiness;quantitativetraitlocus;