水稻籽粒性状的SSR关联分析.doc

水稻籽粒性状的SSR关联分析 陈氏秋江１,２　党小景１　刘强明１　赵凯铭１,３　王卉１　洪德林１, (１南京农业大学作物遗传与种质创新国家重点实验室,江苏南京２１００９５;２越南顺华大学顺华农林业大学农学院,越南顺华;３江 苏省泰州市红旗种业有限公司,江苏泰州２２５３１１;通讯联系人,EＧmail:delinhong＠njau．edu．cn) AssociationAnalysisofRiceGrainTraitswithSSRMarkers TRANThiThuGiang１,２,DANGXiaoＧjing１,LIUQiangＧming１,ZHAOKaiＧming１,３,WANGHui１, HONGDeＧlin１, (１StateKeyLaboratoryofCropGeneticsandGermplasmEnhancement,NanjingAgriculturalUniversity,Nanjing２１００９５,China; ２CollegeofAgronomy,HueUniversityofAgricultureandForestry,HueUniversity,１０２PhungHungStreet,HueCity, Vietnam;３JiangsuHongqiSeedCompanyLtd,Taizhou２２５３１１,China;Correspondingauthor,EＧmail:delinhong＠njau．edu． cn) TRANThiThuGiang,DANGXiaojing,LIUQiangming,etal．AssociationanalysisofricegraintraitswithSSR markers．ChinJRiceSci,２０１４,２８(３):２４３Ｇ２５７． Abstract:Thegeneticdiversity,populationstructureandlinkagedisequilibriumof５４０ricevarietiesfromdifferent ecologicalregionswereassessedwith２６２pairsofsimplesequencerepeat(SSR)markers．Thegenerallinearmodel methodinsoftwareofTASSELwasusedtoanalyzetheassociationbetweenSSRmarkersandfourgraintraits．The mainresultswereasfollows:１)thepopulationwasclassifiedintosevensubpopulations,withlinkagedisequilibriumat certainextentbetweenSSRmarkerlocieitherintraＧchromosomeorinterＧchromosomewithineachsubpopulation． ２)Among４５grainlengthＧassociatedSSRmarkerlocidetectedinallthreeyears,RM５８４９hadthehighestcontribution rate(５２?８６％),RM３７６６Ｇ１４０hadthehighestpositivephenotypiceffect,theircarriervarietywasNongxiang２５． Among７grainwidthＧassociatedSSRmarkerloci,RM１７hadthehighestcontributionrate(６３．６１％),RM１７Ｇ１４５and RM５７３Ｇ２３５hadthehighestpositivephenotypiceffect,thecarriervarietieswereNingjinghui３３８and Zaoshirihuangdao,respectively．Among１１grainthicknessＧassociatedSSRmarkerloci,RM２７６hadthehighest contributionrate(２５．４４％),RM５０８Ｇ２１５andRM７２８８Ｇ２０５hadthehighestpositivephenotypiceffect,carriervarieties werebothHongmangshajing．Amongsix１０００ＧgrainweightＧassociatedSSRmarkerloci,RM１７hadthehighest contributionrate(３２．１７％),RM１７Ｇ１４５hadthehighestpositivephenotypiceffect,the