被毛转拟蜘蛛牵丝蛋白基因克隆羊鉴定本科论文.doc

本科毕业论文 被毛转拟蜘蛛牵丝蛋白基因 克隆羊鉴定 学 院 生命科学学院 专 业 生物科学 年 级 2009 学 号 091114557 姓 名 梁玮 指导教师 孟凡华 职 称 讲师 论文提交日期：二0一三年五月 摘 要 羊毛主要由蛋白质组成。是主要的纺织原料。但由于羊毛的性能主要是由编码羊毛蛋白的基因决定的，所以采用生物工程技术引入优良基因结合动物克隆手段生产转基因克隆动物是改变现状的有效途径。蜘蛛丝的主要成分也是蛋白质，高强度、高弹性、高断裂功、低密度、耐高温，有很高的利用价值。通过转基因技术使蜘蛛牵丝蛋白基因在山羊被毛中特异表达，将能有效改变羊毛的机械性能。本实验以转拟蜘蛛牵丝蛋白山羊胎盘和羊毛为材料，通过对胎盘提取基因组，进一步对其DNA、RNA水平PCR验证以及对转基因羊与自然羊不同部位羊毛的拉力测试。结果显示胎盘DNA、RNA中含有蜘蛛牵丝蛋白基因的片段，并且通过羊毛拉力应力测试仪测的结果显示克隆羊不同部位的羊毛拉力比自然羊的机械性能都好一些。 关键词：蜘蛛牵丝蛋白基因； 克隆羊胎盘； 羊毛；羊毛拉力 Hair turn dragline silk protein gene clone identification Abstract Wool is mainly composed of protein. It is the main textile raw materials. But because the wool performance is mainly determined by the wool protein encoding gene, so the use of biological engineering technology into the excellent combination of animal cloning gene in transgenic animal production is an effective way to change the status quo. The main component of spider silk protein, high strength, high elasticity, high work of fracture, low density, high temperature resistant,has the very high use value. Through transgenic technology makes the spider dragline silk protein gene specific expression in goat hair, will be able to effectively change the mechanical properties of wool. In this experiment, turn the dragline silk protein goat placenta and wool as raw material, through the genome was extracted from the placenta, further to its DNA, RNA level of PCR validation and of transgenic sheep and wild sheep in different parts of wool tensile test. Results showed that placental DNA, RNA containing spider dragline silk protein gene fragment, and the wool pulling force measurement results show pull wool sheep clones in different parts of the mechanical properties than wild sheep is better. Key words: Spider dragline silk protein gene; Wool cloned placenta;Wool; Wool tension 目 录1 1.1羊毛的概述………………………………………………………………………1 1.2蜘蛛牵丝的概述1 1.3转基因技术的发展………………………………………………………………1 1.4蜘蛛丝的应用背景…………………