毕业设计（论文）_软枣猕猴桃果实成熟软化中果胶分解酶活性变化.doc

延 边 大 学 （ 二 ○ 一 ○ 年 六 月 摘 要 为了研究软枣猕猴桃果实成熟软化中果胶分解酶活性的变化，以软枣猕猴桃为试材，测定了软枣猕猴桃在采后多聚半乳糖醛酸酶(Polygalacturonase，简称PG)和(-半乳糖苷酶（β-galactosidase，简称β-Gal酶）活性变化。结果表明，软枣猕猴桃果实在生长发育初期PG酶活性较高，在成熟前活性最低，但成熟期又急剧增加；软枣猕猴桃果实采后1d，PG酶活性达峰值，说明PG酶在软枣猕猴桃采后前期起主要作用。软枣猕猴桃果实在生长发育过程中(-Gal酶活性除幼果期外持续增加；软枣猕猴桃果实采后(-Gal酶活性继续增加，在采后4d达峰值，说明(-Gal酶在软枣猕猴桃采后后期起主要作用。软枣猕猴桃果实成熟软化中果胶分解酶活性的变化，为软枣猕猴桃果实软化机理研究提供重要的基础数据，对软枣猕猴桃资源的开发利用具有非常重要的意义。 关键词：软枣猕猴桃；生长发育；采后；PG酶；(-半乳糖苷酶 Abstract In order to study the changes of pectin decomposition enzyme activity in Actinidia arguta fruit during mature and softening, we used Actinidia arguta fruit as materials，determined the changes of Polygalacturonase enzyme (Polygalacturonase, was called the PG enzyme) and β-galactosidase (β-galactosidase, abbreviation β-Gal enzyme) activity. The results indicated that PG enzyme activity was high in the initial period of growth and lowest before maturity period，but increased largely during maturity period; PG enzyme activity reached a peak in the 1d after picking ，showing that PG enzyme play a leading role in the earlier period after picking. β-Gal enzyme activity increased sostenuto during growth except young fruit stage; β-Gal enzyme activity continue increased after picking，reached the peak in the 4d after picking，showing that β-Gal enzyme play a leading role in the later period after picking.The changes of pectin decomposition enzyme activity in Actinidia arguta fruit during mature and softening providing important basic status for softening mechanism research. It has the very vital significance to the development and use of Actinidia arguta resources. Key words: Actinidia arguta ；Growth；After picking ；Polygalacturonaseβ-galactosidase 目 录 引 言 1 1材料与方法 2 1.1试验材料 5 1.2试验方法 5 2结果与分析 7 2.1 软枣猕猴桃果实生长发育过程中PG酶活性变化 7 2.2 软枣猕猴桃果实采后PG酶活性变化 7 2.3 软枣猕猴桃果实生长发育过程中(-半乳糖苷酶活性变化 8 2.4 软枣猕猴桃果实采后(-半乳糖苷酶活性变化 8 结 论 10 参考文献 11 致谢词 13  引 言 软枣猕猴桃(Actinidia arguta Sieb.et Zucc)，又名软枣子，猕猴梨，藤瓜,属于猕猴桃科(Actinidiaceae)、猕猴桃属(Actinidia)多年生落叶藤本植物，是猕猴桃属中