必威体育精装版德国美天妮磁珠分选.ppt

MACS技术设备与试剂 MACS微珠： MACS 微珠是与高度特异性单克隆抗体相偶联的超顺磁化微粒，可用于目的细胞的磁性标记。 特性： 50nm大小 对细胞没有毒性 可生物降解 不影响细胞特性 MACS微珠 MACS技术设备与试剂 MACS分选柱： 》填充有不同规格的铁珠。 》铁珠表面有亲水包被，不损伤细胞。 》无菌包装。 》可用于分选多种细胞及亚细胞物质、 细菌、病毒、mRNA和蛋白质。 专利产品 将磁场扩大1000－10000倍——高纯度，高回收率 MACS技术设备与试剂 MACS分选柱： MACS技术设备与试剂 MACS分选器：从手动到自动，从实验室到临床 MACS技术设备与试剂 MACS分选器 MACS? 细胞因子分泌细胞分析与分选 New 人类细胞因子分泌细胞分选与检测试剂盒 IFN-g 分泌细胞 IL-2分泌细胞 IL-4分泌细胞 IL-10分泌细胞 TNF-?分泌细胞 MSC Research Tool Box - CD271 (LNGFR)new 成分: Cytokine Secretion Assay is a completely new technology (got a different colour on the slide). Analysis and also enrichment of extremely rare antigen-specific T cells, for example. Cytokine Secretion Assay is a completely new technology (got a different colour on the slide). Analysis and also enrichment of extremely rare antigen-specific T cells, for example. Antigen-specific restimulation leads to cytokine secretion Catch reagent forms an artificial surface to catch the cytokine which is secreted. Catch reagent is composed of two antibodies, one of them directed against the cytokine. Labeling with detection antibody. Indirect magnetic labeling is optional for enrichment. Without enrichment (no columns): detect frequencies between 0.01-0.1% of total cells. With enrichment: detect frequencies down to 0.0001 % (1 cell in 10e6 cells!!) In addition, cells stay alive during enrichment and detection and can be taken into culture or used for molecular analysis. There are a lot of different separation techniques apart from Magnetic Cell Sorting. Some of them are also used to pre-enrich cells befor performing a megnetic cell sorting. There are a lot of different separation techniques apart from Magnetic Cell Sorting. Some of them are also used to pre-enrich cells befor performing a megnetic cell sorting. Cytokine Secretion Assay is a completely new technology (got a different colour on the slide). Analysis and also enrichment of extremely rare antigen-specific T cells