蛋白芯片的应用精要.ppt

（6）ISL1 Expression Resulted in Elevated Expression of MCP3 in hMSCs Using human angiogenesis antibody arrsay to establish the involvement of cytokines secreted from ISL1-hMSCs in enhancing the angiogenic capacity of HUVEC qRT-PCR analysis of ISL1-hMSCs and control hMSCs ISL1-hMSCs条件培养基显著增加了MCP3的表达 qRT-PCR analysis of ISL1-hMSCs and control hMSCs ISL1-hMSCs条件培养基还显著增加了细胞外粘附因子ICAM1的表达 In Matrigel plug assays, the tube formation ability of human umbilical vein endothelial cells (HUVECs) grown in CM from ISL-hMSCs cultures was significantly impaired by addition of an anti-MCP3 antibody 中和试验添加MCP3抗体，降低了ISL1-hMSC条件培养基中HUVEC小管形成 Test the impact of secreted MCP3 on the biological properties of HUVECs using a neutralizing antibody against MCP3 中和试验添加MCP3抗体，降低了ISL1-hMSC条件培养基中细胞存活 HUVECs survival in CM from ISL1-hMSCs cultures, with and without the addition of anti-MCP3 neutralizing antibody In transwell assays, the percentage of migrated HUVECs in CM from ISL-hMSCs cultures was significantly impaired by addition of an anti-MC3 antibody 中和试验添加MCP3抗体，降低了ISL1-hMSC条件培养基中HUVEC细胞迁移 The Cytodex-3 Beads-based tube formation assay 中和试验添加MCP3抗体，降低了ISL1-hMSC条件培养基中HUVEC芽和腔形成能力 Cardiomyocytes Endothelial cells Smooth muscle cells Phenotype Self-renewal Multipotency Survival Migration Conclusion 目前，本文的研究结果提供了ISL1介导的血管生成分子机制方面的新见解。由于hMSC 具有分化潜能、造血支持、免疫调控和自我复制等特点，其作为细胞疗法用于再生医学和在临床研究有很大潜能。因此我们的研究结果可以为研究缺血性疾病的治疗有重要意义。 Thinking 1、Experimental design 2、Methods staining、expression of protein、immunofluorescence 3、Our molecular biology laboratory services ELISA (especially Cell-Based ELISA) Western Blot ...... 4、Our products Human cytokine antibody array Protein expression antibody array ...... Thanks for your attention. Thanks for your attention Islet-1 Overexpression in Human Mesenchymal Stem Cells Promotes Vascularization Through Monocyte Chemoattractant Protein-3 Major： Biochemistry and Molecular Biology Date：2014.12.19 Contents Conclusion 5 Introduction 2 Materials and methods 3 R