酶法提取花生蛋白响应面回归研究.pdfVIP

2010 First International Conference on Cellular, Molecular Biology, Biophysics and Bioengineering (CMBB ) Optimization of Enzymatic Extraction of Peanut Protein by Response Surface Methodology 1 2* 2 Yan Hong Lv , Shao Bing Zhang , Zhou Tang 1. School of Biological Engineering 2. School of Food Science and Technology Henan University of Technology ZhengZhou 450052, Henna,China *Corresponding author. Email: shaobingzhang@126.com Abstract—Four kinds of proteases including Alcalase 2.4L,2709 alkline protease, AS1398 neutral protease and Protex 6L were used to extract peanut protein, respectively. 2709 alkline protease showed the highest extraction efficacy, followed by Protex 6L. Response surface methodology (RSM) was used to study the effects of 2709 alkline protease on extraction of peanut protein. The highest protein yield was 94.3% under the optimal conditions (solid-to-water ratio 1:4, alkaline pH 9.0, enzyme amount 3.75g (in 60g of peanut seeds) and hydrolysis time 1.5 h. By analysis of the experimental results, a suitable model was obtained for predicting the yield of protein (R2 = 0.8182 ，p0.01). The solid-to-water ratio highly significantly (p0.01) affected the protein yield. Keywords- enzymatic extraction; protease; peanut protein; response surface methodology 酶法提取花生蛋白的响应面回归研究 1 2* 2 吕燕红 ，章绍兵 ，唐舟 （河南工业大学 1 生物工程学院 2 粮油食品学院，河南 郑州 4500