《主动载药法制备硫酸长春新碱脂质体及其包封率的测定》.pdf

主动载药法制备硫酸长春新碱脂质体及其包封率的测定 摘要：目的制备硫酸长春新碱脂质体，建立包封率测定方法。方法采用pH梯度法制备硫酸长春新碱脂质体；以阳离子交 换树脂分离脂质体和游离药物；用RP-HPLC测定脂质体的包封率。结果硫酸长春新碱脂质体包封率大于85％；在所建立的 色谱条件下硫酸长春新碱与辅料及溶剂峰分离良好；硫酸长春新碱在1．0。12．5nag·L-1内线性关系良好(r=0．9999)；精密度 和回收率均合格。结论pH梯度法适于制备硫酸长春新碱脂质体，所建立的分析方法准确可靠，简单快速，可以用于硫酸长 春新碱脂质体包封率的测定。 关键词：pH梯度法；硫酸长春新碱；脂质体；高效液相色谱法；包封率 中图分类号：R943．41 文献标识码：A 文章编号：1001—2494(2005)20—1559—04 of vincrisline and ofits effi- sulfateactive determination Preparationliposomesentrapped by loading entrapment ciency ZHAO Yahl，一，YUBinl，DENG Yi—huiH，XIAO“．yil】91(1．School矿‰mw，Shenyang Sciences，ChinaMedical 110001，China) 110016，China；2．CollegeofBasicMedical University，Shenyang To vincristinesulfate andto amethodfor its efficien- AK．qlliAcr：OBJECTIVE prepare liposomesdevelop determiningentrapment was inthe vineristine Vineristine the remote cy．MEqMODS encapsulatedliposomesusingpHgradient-dependentloadingtechnique．Free and were cation resinandRP-HPLCWas to the Theen- liposomesseparatedby exchange determine applied entrapmentefficiency．蛐．SULTS ofvineristinesulfate methodexceeded85％．TheRP-HPLCmethodwasvalidatedand gradient trapmentefficiency liposemespreparedbypH WaS standardcu．uvewas of withthe vincristinesulfatewell linearoverthe 1．0～12．5 correlationcoefficientof separated．The range mg·L一1 0．999 The issuitablefor sulfate methodestablishedinthis gradi