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Published January 10, 2011
Article
High prevalence of low affinity peptide–
MHC II tetramer–negative effectors during
+
polyclonal CD4 T cell responses
1 2 2 1
Joseph J. Sabatino Jr., Jun Huang, Cheng Zhu, and Brian D. Evavold
1Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322
2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332
T cell affinity for antigen initiates adaptive immunity. However, the contribution of low
affinity cells to a response is unknown as it has not been possible to assess the entire
D
e affinity range of a polyclonal T cell repertoire. In this study, we used a highly sensitive two-
n o
i dimensional binding assay to identify low affinity cells in polyclonal autoreactive and w
c
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