橡胶工业有机废水降解微生物的驯化筛选和降解条件研究.pdf

INDUSTRIALWATER WASTEWATER 工皿用水与康水 Vol.37 No.5 Oct.，2006 橡胶工业有机废水降解微生物的驯化筛选与 降解条件研究 顾韬’,2，李捍东 ，‘王平 “ (1.中国环境科学研究院，北京 100012;2.中南林业科技大学 生命科学与技术学院，长沙 412004) 摘要:用含M盐(2一硫醇基苯并噬咬)的橡胶工业有机废水驯化活性污泥，筛选出8株菌。纯化后进行混合培 养，对其生长条件进行分析后发现:复合菌适合在30一35℃的偏碱性条件下生长，能耐受50g/L盐度的影响。 当添加葡萄糖和尿素后对复合菌生长影响较大，同时可使橡胶工业有机废水降解率分别提高18.6%和7.56%;无 机离子也对复合菌的降解能力有一定的促进作用。采用综合正交试验得出:葡萄糖、尿紊、菌量、转速分别为2 g/L,1g/L,20%,80r/min为复合菌的最佳降解条件，最高降解率为68.24%，从而提高了投菌法的降解效率。 关键词:橡胶工业有机废水;驯化筛选;生物降解 中图分类号:X703.1 文献标识码:A 文章编号:1009-2455(2006)05-0054-05 Researchonacclimationandscreeningofmicroorganismfor. degradationoforganicwastewaterfromrubberindustryand degradationconditionsthereof GuTao，LIHan-don扩，WANGPing (1.ChineseResearchAcademyofEnvironmentalScience,Beijing100012,China;2.SchoolofLfieScienceand Technology,CentralSouthUniversityofForestandTechnology,Changsha412004,China) Abstract:8strainsofbacteriawereisolatedfromtheactivatedsludgeacclimatedbyMsalt(2-mercaptobenzo- thiazole)-containingorganicwastewaterfromrubberindustry.Thebacteriaweremixedcultivatedafterthepurifica- tion,throughanalyzingtheirlivingconditions,itwasfoundthat:compoundbacteriaadapttoalkalescentconditonat 30一35℃，andtolerate50留LNaCl.Addingglucoseandcarbamidegreatlyinfluencedthegrowthofthecompound bacteria,andincreasedthedegradationrateofthewastewaterfromrubberindustryby18.6%and7.56%respec- tively;inorganicionscaneffectivelyimprovetheperformanceofthecompoundbacteriaonwastewaterdegradation, theresultsofthecomprehensiveorthogonalexperimentshowedthat,thehighestdegradationrateofwastewaterby thecompoundbacteriawas68.24%whichcouldbeobtainedwhenthemassconcentrationofglucose,carbamid