甜高粱蔗糖合酶表达和蔗糖积累的相关分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(1): 185− 189 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.00185 甜高粱蔗糖合酶表达与蔗糖积累的相关分析 杨 明1,3 刘丽娟1 李莉云1 王 博1 常金华2 刘国振1,* 1 ; 2 , 071000; 3 , 050015 : , , Western blotting (sucrose synthase, SS) , , SS ( ), () , 2 , 2 SS , , : ; ; ; Western blotting Correlation Analysis between Sucrose Synthase Expression and Sucrose Accumulation in Sweet Sorghum [Sorghum bicolor (L.) Moench] YANG Ming 1,3, LIU Li-Juan 1, LI Li-Yun 1, WANG Bo 1, CHANG Jin-Hua 2 , and LIU Guo-Zhen 1,* 1 College of Life Sciences, 2 College of Agronomy, Agricultural University of Hebei, Baoding 071000, China, 3 North China Pharmaceutical Group Corporation, Shijiazhuang 050015, China Abstract: Alcohol production by using sweet sorghum [Sorghum bicolor (L.) Moench] might be a potential way to provide bio-energy in the future, so it is necessary to learn about the mechanism of sucrose accumulation in sweet sorghum for exploita- tion of bio-energy. The experiment was conducted using two sweet sorghum varieties, to detect expression of sucrose synthase (SS) in leaves and stems by using Western blotting, and to analyse the correlation between SS expression and sucrose accumula- tion. The results indicated that the SS expression in sweet sorghum leaves and stems was lower at early growth stages (jointing and heading stage), increased dramatically at late growth stages (flowering, grain filling, and dou