体外循环不同氧压对红细胞衰变加速因子影响的临床研究.pdf

体外循环不同氧分压对红细胞衰变 加速因子影响的临床研究 摘 要 加速因子(decay．accelerating 入手术室建立静脉通道后行有创动静脉穿刺，快速诱导后气管内插管，静吸复合麻醉。 氧分压控制在300-500 放15 O．2ml酵母菌加入lI管，然后将I管、II管放入374C温箱中孵育60min；用小鼠抗人 CD55 FITC荧光单克隆抗体直接标记，用流式细胞仪检测红细胞上CD55表达(I管 与B组相比，非紫绀型心脏病C组与D组相比病人的年龄、体重、CPB时间、主动 活性及数量，无统计学意义。结论：CPB中高氧分压可使紫绀型心脏病病人的红细 胞CD55活性明显增强，缺氧红细胞可能存在再氧合损伤：控制氧分压对红细胞免疫 功能有一定的保护作用。 关键词：体外循环；不同氧分压；红细胞CD55；再氧合损伤 4 Theclinical different ofeffectsof tensionon study oxygen factorinthe CPB erythrocyte decay—accelerating during effectsofdifferent tensionon Abstract：Objective：To Decay investigate oxygen factorinthe accelerating erythrocyteduring were dividedintofour with patientsrandomly groups：Thehyperoxicgroup cyanotic heart congenitaldisease(groupA，n=10)andhypoxic B，n=10)；The group(group with heart hyperoxic C，n=1 groupacyanoticdisease(group0)andhypoxicgroup(group D，n=t accessandartedovenousinvasive weredoneonfour o)．Intravenous puncture of inthe whichendotrachealintubationwith room，in groupspatients operating after was Anesthesia—inhalationinduction．CPBestablishedafterthemedian rapid tensioninthe Wascontrolled sternotomy．Oxygenhypoxicgroup on and