高效液的相色谱测定小蔓长春花中长春胺的方法优化.pdf

,2010,30( 12) : 2555- 2560 A cta Bot. Bor eal. Occid ent . Sin. :2010) * 侯恩太, 李多伟 , 王英娟, 倪士峰, 陈千良 ( , 710069) : 1%(pH 7.5)1%1% ( pH 7.5)1% ,, ,: , , ;6535 ,, 2 , 0. 05~ 0.5 g/ L ( R = 0.997) , 100. 71%(n= 5) ,RSD= 2.23% , 1%( 6535, pH 7. 5) , :; ;; :Q503 :A Optimization of HPLC Determination Method for Vincamine in Vinca minor L. * H U Entai,LI Duowei ,WANG Yingjuan,NI Shifeng,CHEN Qianliang (College of Life Science, Northwest University, Xi an 710069, China) Abstract: Utilized different proportion of methanol1% diethylamine (pH 7.5) , methanol0.01 mol/ L am monium carbonate,acetonitrile1% diethylamine ( pH 7. 5) as mobile phase. Vincamine retention time and separation conditions were researched and chosen the best mobile phase for vincamine determination.Re sults showed: In methanolammonium carbonate, acetonitrileammonium carbonate, acetonitrilediethyl amine system,vincamine was difficult to completely separate.The best mobile phase was methanol1% di 2 ethylamine (pH 7.5) ( 6535, V/ V) . Vincamine within 0.05~ 0.5 g/ L had a good linearity (R = 0.997) . The average recovery was 100. 71% ( n= 5) , with RSD= 2. 23% . Research shows that this mobile phase system can be used for quality detection of V.minor L. . Key words: Vinca minor L.;vincamine;RPHPLC ;mobile phase ( Vinca minor L.) , :( vincamine) , (apovin [2] ( Vinca L.) , c