一种用的于中药纯化过程的近红外光谱分析新方法.pdf

2003 年第 61 卷 化 学 学 报 Vol. 61 , 2003 第 5 期 , 742～747 ACTA CHIMICA SINICA No. 5 , 742 ～747 一种用于中药纯化过程的近红外光谱分析新方法 杨南林 　　程翼宇 　　瞿海斌 (浙江大学药学院　杭州 310027) 摘要 　针对中药大孔吸附树脂纯化过程缺乏在线检测分析手段等问题 ,提出基于光纤近红外透射光谱的过程分析新方法. 以黄连提取物为例 ,采用 HPLC 分析测定值作参比 ,用偏最小二乘算法建立了近红外透射光谱校正模型 ,并成功地用于预 测黄连生物碱在大孔树脂纯化过程中的洗脱曲线. 本方法实时、快速 ,可同时测定洗脱物中盐酸小檗碱 、盐酸巴马亭 、盐酸 药根碱和黄连总生物碱的浓度 ,预测精度满足工业过程分析要求 ,为提高中药质量分析水平提供了新的途径. 关键词 　过程分析化学 ,近红外透射光谱 ,偏最小二乘算法 ,中药分析 ,黄连 ,大孔吸附树脂纯化 An Approach to Purifying Process Analysis of Chinese Herbal Extracts Using NIRS YANG, NanLin 　　CHENG, YiYu 　　QU , HaiBin ( ) College of Pharmaceutical Science , Zhejiang University , Hangzhou 31002 7 Abstract 　A new method for process analysis of the production of herbal medicine , which is based on fiber optic near infrared transmission spectroscopy (NIRTS) is developed. The purification process of Coptis chinensis extracts with nonionic macroreticular resin was selected as an example. HPLC was used to determine the concentrations of some alkaloids for reference. Four calibration models were then developed to correlate NIRTS spectra with the concentration values of the alkaloids determined by HPLC using PLS algorithm. The models were applied to successfully predict the elutio