PAMAM_阿霉素口服释药系统构建_cfb7158d_da1c_4265_882.pdfVIP

PAMAM- -(PAMAM) PAMAM PAMAM- MTT PAMAM Caco-2 Caco-2 Doxorubicin-PAMAM PAMAM P A Doxorubicin-PAMAM PAMAM PAMAM Doxorubicin-PAMAM PAMAM PAMAM PAMAM Caco2 Abstract: Object: PAMAM is reviewed as a newly coming macromolecule which has been demonstrated potential use as a drug delivery system. This article will discuss the preparation of Doxorubicin-PAMAM, measuring the Tran epithelial transport and protein uptaking rate. Method: We prepared Doxorubicin-PAMAM complex and then comparing the releasing rate of Doxorubicin alone with Doxorubicin-PAMAM complex. After examining the cytotoxic activity, we carried through the concentrate-cost and the time-cost experiment among Doxorubicin, Doxorubicin-PAMAM and Doxorubicin-Cyclosporine A. In the end, we examined the rate of fluorescently labeled Doxorubicin-PAMAM uptake and transport in vitro and in vivo. Result: PAMAM can effectively embed Doxorubicin and the incorporation 1499 efficiency is quite high while the release rate is controlled. PAMAM itself has no cytotoxic activity to cells. The complex has a notable advantage in transport and uptake. Conclusion: PAMAM, as a macromolecule drug delivery system, has a remarkable benefit to the delivery of their drug and modulate their release. Key words: PAMAM, Doxorubicin, Caco2 cells, oral bioavailability P P4503A