龙漫远细胞生物学重点分析.ppt

Numbers of detected query–subject pairs in the BLASTP analysesQuery (strand) Human Mouse Frame 1 (+) 430 65 Frame 2 (+) 467 69 Frame 3 (–) 39 14Frame 4 (–) 14 3 Frame 5 (–) 40 21 Total 990 172 What TEs are involved? Lorenc and Makalowski, 2003, Genetica, 118: 183-191 Bergthorsson et al., 2003, Nature, 424: 197-201 Horizonatal transfer of plant genes (among flowering pllants): Gene fusion : What mechanisms? Wang et al., 2004, Nature Genet ? B: D. mauritiana mau-mkgp1, 3L, 60C11 mau-mkgp2, 3L, 60C11 829bp ATG Parental copy TAA ATG TAG ATG TAG mau-mkgr3a, X, 5A mau-mkgr3b, X, 5A ATG ATG TAG ATG polyA polyA polyA D. melanogaster mkgp, 3L, 66C D. mauritiana new mkgp mkgr3, X, 5A mkgr2, X, 9A mkgr, X, 9B New copy 2a New copy 2b New copy 1a New copy 1b New copy 1c In D. mauritiana: N-domain C-domain Wang et al., 2004, Nature Genet D. melanogaster (mel) D. simulans (sim) D. yakuba (yak) D. erecta (ere) D. ananasse (ana) 3 mys 8 mys 12 mys 30 mys Origination de novo: 5 testis genes originated from non-coding regions in Drosophila: Levine et al., 2006, PNAS. 103: 9935 Reading frame shifts Serotonin receptor: (HTR3D) Olfactory receptor (OR3a family) ~1000 human genes, But computational and Preliminary, more biological expriments needed Raes and Van de Peer 2005, Trends Genet Okamura et al., 2006, Genomics frame shift mutation as a mechanism to generate new proteins: Okamura K. et al., 2006, Genomics Sequences producing significant alignments: (bits) Value NM_012314 157 1e-38 NM_178228 157 1e-38 NM_012314 Length = 239 Score = 157 bits (396), Expect = 1e-38 Identities = 75/89 (84%), Positives = 80/89 (89%) Query: 158 TIYPRRGRPMNVGSLQGPRSTEHSRPTFLWALPPKEEPTDASALSMTLPTSGQSQVTHCL 217 +IYP +GRPMNVGSLQ STEHSRPTFLWALPP E PTDASALS+TLPTSGQ++V HCL Sbjct