毕业论文（参考）中国黄牛亲缘关系分析.docVIP

中国黄牛mtDNA cyt b基因进化分析 中国黄牛mtDNA cyt b基因进化分析 生物学实验教学中心 目 录 引言 1 1 实验材料 4 2 实验方法 4 2.1血样采集和DNA样品制备 5 2.2 PCR引物 6 2.3 PCR反应和PCR产物检测 7 2.4 PCR产物回收测序 8 2.5数据分析与结果处理 8 3 结果与分析 10 3.1中国黄牛基因组DNA提取结果。 10 3.2中国黄牛mtDNA cytB基因PCR扩增结果。 11 3.3 中国黄牛mtDNA cytB基因序列分析结果。 16 3.4 中国黄牛mtDNA cytB分子进化分析，进化树的构建。 17 总结 18 参考文献 18 中国黄牛mtDNA cyt b基因分子进化分析 Chinese ox mtDNA cyt b gene evolution analysis ???? zhanjing ?? （Tutor：Zhangrunfeng） （College of Life Sciences , Hubei Normal University, ?Huangshi, 435002） Abstract: Using molecular biological methods, combined with bioinformatics, the use of mitochondrial DNA sequence analysis and genetic relationship of Chinese cattle origin and evolution. Cattle multiple countries recognized that while at home and abroad, but also faced cattle breeds in the protection of genetic resources, so as soon as possible at the molecular level inherent in cattle breeds in China and the origin of the genetic relationship is very important to study evolution. Determination of the Qinling Mountains north of the cattle, Bohai Black, Mongolian beef, cattle, and cattle Xuanhan south of the Qinling Mountains, southern cattle, seven varieties of Nanyang cattle beef cattle and V00654 mitochondrial DNA cytochrome b gene fragments, 18 of which sites of mutation detection to analyze the base composition and variation, and V00654 cattle standard, application DnaSP Version 3.5 software [214] analysis of all sequence variation (polymorphism) sites, application MEGA version 4.1 progarm calculated breeds and between species based on Kimuras two-parameter, the average genetic distance and the application of MEGA version 4.1 software to build all the haplotypes based on Kimuras two-parameter, the average genetic distance of the NJ and UPGMA phylogenetic tree, at the molecular level from maternal genetic point of view 7 to clarify the origin of differentiation between cattle breeds. The results showed that, V00654 Nanyang cattle and beef cattle