染色体非整倍性检测新方法.pptVIP

* Rapid sequencing, de novo assembly identification of novel microbial strains. Monday May 30* Library preparation O104:H4 and HUSC41 samples (reference) strain libraries prepared TuesdayMay 31 Sequencing runs 0104:H4 amplified and sequenced 2 x 2 runs (Ion 314) WednesdayJune 01 Sequencing runs 0104:H4 sequenced 3 x 2 runs (Ion 314) ThursdayJune 02 Assembly Draft Genome identified, Assembled, Submitted and Released from NCBI FridayJune 03 Assay Design TaqMan Assays Designed *May 22 CEDC reports significant increase in patients with hemolytic uremic syndrome “The biggest advantage [of the PGM] from my point of view as a public health official is that its speedy, and speed is what is needed at the moment,” Prof. Dr. Med Dag Harmsen, University Hospital Muenster “[The PGM] takes the shortest time to generate genomic data.” Junjie Qin, BGI Monday May 30* Library preparation O104:H4 and HUSC41 samples (reference) strain libraries prepared TuesdayMay 31 Sequencing runs 0104:H4 amplified and sequenced 2 x 2 runs (Ion 314) WednesdayJune 01 Sequencing runs 0104:H4 sequenced 3 x 2 runs (Ion 314) ThursdayJune 02 Assembly Draft Genome identified, Assembled, Submitted and Released from NCBI FridayJune 03 Assay Design TaqMan Assays Designed 快速未知病原筛查— E. coli Outbreak Characterized using Ion PGM? sequencer in 3 days * PGD, 无创产前，单基因病 22条染色体全部可以检测 Panel is designed to screen more than 100 mendelian inherited diseases for known mutations Panels are designed to detect nearly all exons for each gene in the assay Using Ion 316 Chips the whole panel can be analyzed with a single run The panel covers several categories of disease states ranging from cardiovascular to neuromuscular diseases Average amplicon size is expected to be approx 170bp, therefore 200bp template and sequencing chemistry should be used Amplicons in general are designed to minimize coverage of the 5’UTR of the exons * Confidential and Proprietary—DO NOT DUPLICATE Confidential and Propriet