铅对拟南芥叶绿体、线粒体膜电位和活性氧自由基的影响研究解说.doc

SHANDONGＵＮＩＶＥＲＳＩＴＹ　ＯＦ　ＴＥＣＨＮＯＬＯＧＹ 毕业论文 铅对拟南芥叶绿素、线粒体膜电位 和活性氧自由基的影响研究 学 院： 生命科学学院 专 业： 生物工程 学生姓名： 刘松鹤 学 号： 0812107567 指导教师： 刘东武 2012年中文摘要 重金属铅（Pb）是植物生长发育过程中的非必需元素，它主要在植物的根部被吸收并积累，进而会对植物的光合作用产生抑制作用，从而影响到植物的生长发育。 拟南芥因为具有生长周期短、种子多、基因组小（植物基因组中最小的）、生命力强的优点，被广泛用于植物遗传学、发育生物学和分子生物学的研究。拟南芥是在植物科学，包括遗传学和植物发育研究中的模式生物之一。 本项研究对拟南芥叶绿体、线粒体、根部的活性氧自由基分别进行荧光染色标记，采用激光扫描共聚焦显微镜技术，研究五个不同浓度的重金属铅随着时间的推移对拟南芥叶绿体、线粒体以及根部的活性氧自由基的影响。0.025g/L、0.05g/L，0.1g/L，0.25g/L，0.5g/L通过本项研究，揭示重金属铅对拟南芥叶绿体、线粒体膜电位和活性氧自由基的作用规律，从而更好的服务于对拟南芥的培养、研究，从而服务于农业生产。 关键词：铅拟南芥叶绿体线粒体活性氧自由基 Abstract The heavy metal lead (Pb) is a non-essential elements in plant growth and development, it mainly in the root of the plant is absorbed and accumulated, and then will plant photosynthesis inhibition, thus affecting to plant growth. Because the Arabidopsis have growth cycle is short, the more seeds, genome small ( the least of the genome in plants), the advantage of strong vitality, is widely used to plant genetics and molecular biology, and developmental biology. Arabidopsis in plant sciences, including one of the model organisms in genetics and plant development. The study of arabidopsis chloroplasts, mitochondria, the active oxygen free radicals are fluorescent marker dye, use the confocal laser scanning microscopy techniques, the five different concentrations of heavy metal lead with the passage of time of arabidopsis chloroplasts, mitochondria and root of reactive oxygen species the effects of free radicals.The results of the study show that, the selection of laboratory is 0.025g/L, 0.05g/L, 0.1g/L, 0.25g/L, 0.5g/L five kind of concentration of lead ions, 0.025g/L concentration promote chloroplast chlorophyll and four other kind of concentration is the inhibition; 0.1g/L concentration leaves the mitochondrial membrane potential to promote, four other kind of concentration is the inhibition; Five kinds of concentration of lead ions to root the mitochondrial membrane potential and