金福菇多糖降解工艺优化.docxVIP

安徽大学江淮学院 本科毕业论文 题　　目：　　金 福 菇多糖降解工艺优化　　　　　　　　　　　 学生姓名：　　陈晨　　　 学号： 系 别： 理工部 　　　　专业：　　生物信息技术 　　　　 入学时间：　 　　2012　　　　年　　　09　　　　月 导师姓名：　　陆永明　　 　　 职称/学位：　 讲师　 　 导师所在单位：　　 安徽大学生命科学院 　　　　 　　　　　　 完成时间：　 　　　2016　　年　　　　04　　　　月 金福菇多糖降解工艺优化 摘要 目的: 研究金福菇多糖的最优降解条件。方法：通过新型的制备工艺对金福菇进行粉碎并利用高压均质得到粗多糖，并且通过季铵盐沉淀法得到金福菇多糖TLH-1，然后对金福菇多糖TLH-1进行盐酸降解，针对降解温度、时间、pH等因素进行单因素实验分析，设定三个参数为考察因素，分别为提取时间、提取温度、pH，各取3水平进行正交试验。结果：实验结果表明，各提取因素对金福菇多糖降解片段得率的影响为:以降解时间为主，温度次之，pH最小。所得最佳提取条件：降解时间为80min，降解温度为90℃，pH为5。结论：在合适条件下，盐酸降解能有效降低金福菇多糖的分子量，进而可能影响其生物活性。 关键词 ：金福菇 ；多糖 ；降解 ；工艺优化 Optimization of degradation conditions of polysaccharide from Tricholoma lobayense Heim Abstract Objective: To study the optimal conditions for degradation of polysaccharide from the Tricholoma lobayense Heim . Methods: Polysaccharide TLH-1 from Tricholoma lobayense Heim was obtained by high-pressure homogenization and quaternary ammonium salt precipitation with ultra?ltration. Then the degradation of polysaccharide TLH-1 by hydrochloric acid was carried out and the influencing factors were examined in detail. The main factors, such as temperature, time and pH, were optimized by single factor design and orthogonal experiment.. Results: The experimental results showed that the extraction factors influencing the polysaccharide degradation with the order of time, temperature and pH. The optimum degradation conditions were 80 min for time, 90 ℃ for temperature, and 5 for pH. Conclusion: Degradation of polysaccharide TLH-1 by hydrochloric acid was an effective method to reduce its molecular weight, which might affect its corresponding bioactivities. Keywords：Tricholoma lobayense Heim；polysaccharide; Degradation; Process optimization 目录  TOC \o 1-3 \h \z \u  HYPERLINK \l _Toc449384640 1.引言  PAGEREF _Toc449384640 \h 6  HYPERLINK \l _Toc449384641 1.1多糖简介  PAGEREF _Toc449384641 \h 6  HYPERLINK \l _Toc449384642 1.2低聚糖的功能与获得方法  PAGEREF _Toc449384642 \h 6  HYPER