铅离子胁迫对丹参幼苗生理活性的影响教程.doc

本科生毕业论文（设计） 题 目 铅离子胁迫对丹参幼苗生理活性的影响 专 业 生 物 技 术 院 部 生命科学与技术学院 学 号 姓 名 xxxxxxxxxx 指 导 教 师 xxxxxxxxxxxxxxxxxx 答 辩 时 间 xxxxxxxxxx 论文工作时间：2011年 9 月 至 2012 年 5 月铅离子胁迫对丹参幼苗生理活性的影响 学 生：xxxxxx 指导教师：xxxxxx 摘 要：400mg/L以上)铅对植物的伤害明显。 关键词：丹参；铅；酶；叶绿素 Effect of Pb2+ stress on physiological indices of salvia miltiorrhiza Undergraduate:WangJunlong Supervisor: LuoMinghua Abstract: In the paper, the Salvia miltiorrhiza which are planted in Zhongjiang County area in Deyang, Sichuan province were used as eaperimental materials. The moisture content, content of chlorophyll ,content of proline, content of MDA and activity of peroxidase(POD) of the Salvia miltiorrhiza is studied under different levels of lead stress in this paper. At last, the results display as follows: With the growth of stress time and the concentration of lead, the moisture content will decrease. At low lead concentrations, chlorophyll content will increase in a small degree; at the high lead concentrations, the chlorophyll content will deacrease in a large scale. At low lead concentration, the content of proline, content of MDA and activity of peroxidase will increase with the growth of stress time and stand in a stable level relatively; at high lead concentration, those will increase quickly. The results showed that the low concentration of lead can promote the growth of plants, however, the high concentration of lead damage the plants obviously. Key words: Salvia miltiorrhiza; lead; POD; chlorophyll 目 录 1前 言 1 1.1丹参的研究现状 1 1.2试验目的 1 1.3试验意义 1 2试验材料 1 2.1试验样品 1 2.2主要仪器及试剂 1 2.3主要试剂的配置 2 3试验方法 2 3.1试验流程 2 3.2试验步骤 2 3.2.1丹参的种植及胁迫 2 3.2.2测定含水量 2 3.2.3叶绿素含量的测定 3 3.2.4脯氨酸含量的测定 3 3.2.5丙二醛的含量测定 4 3.2.6酶活性的测定 5 4结果及分析 5 4.1含水量的变化 5 4.2叶绿素含量的测定结果 6 4.3脯氨酸含量的测定结果 8 4.3.1脯氨酸的标准曲线 8 4.3.2脯氨的测定结果 8 4.4丙二醛含量的测定结果 9 4.5酶活性的测定结果 10 5讨论 10 6结论 11 7本实验的不足之处 11 参