CMIChapterTCR说课.ppt

第五章 内容小结 1. 抗体及TCR多样性的形成机制。 The end of chapter 5 Cellular and Molecular Immunology Xian-Hui He Department of Immunobiology Jinan University 2014-10 Chapter 5 The Generation of Lymphocyte Antigen Receptors 淋巴细胞抗原受体的形成 Primary immunoglobulin gene rearrangement. T-cell receptor gene rearrangment. Structural variation in immunoglobulin constant regions. Secondary diversification of the antibody repertoire. Evolution of the adaptive immune response. (略) Part II Antigen recognition 1. Primary immunoglobulin gene rearrangement. 免疫球蛋白基因的重排 Primary immunoglobulin gene rearrangement. 5-1 Immunoglobulin genes are rearranged in antibody-producing cells. (免疫球蛋白基因只在产生抗体的细胞中重排) This process of rearrangement is known as somatic recombination (体细胞重组). Primary immunoglobulin gene rearrangement. 5-2 Complete genes that encode a variable region are generated by the somatic recombination of separate gene segments. (分离的基因片段体经细胞重组产生编码可变区的全部基因) 5-2 Complete genes that encode a variable region are generated by the somatic recombination of separate gene segments. Primary immunoglobulin gene rearrangement. 5-3 Multiple contiguous V gene segments are present at each immunoglobulin locus (基因座位). (在每一个免疫球蛋白基因座位存在多个连续的V基因片段) genetic loci - the ?, ?, and heavy-chain loci Primary immunoglobulin gene rearrangement. 5-4 Rearrangement of V, D, and J gene segments is guided by flanking DNA sequences. (两侧DNA序列指导V、D、J基因片段的重排) recombination signal sequences (RSSs): 重组信号序列. VDJ重组遵循12/23 rule. Primary immunoglobulin gene rearrangement. 5-5 The reaction that recombines V, D, and J gene segments involves both lymphocyte-specific and ubiquitous DNA-modifying enzymes. (VDJ重组涉及淋巴细胞特异性和非特异性DNA修饰酶) 淋巴细胞特异性：V(D)J recombinase: RAG-1 (RAG1) and RAG-2 (RAG2) DNA repair: DNA-dependent protein kinase (DNA-PK) DNA ligation: DNA ligase IV 切割 修复-添加 连接 Primary immunoglobulin gene rearrangement. 5-5 The reaction that recombines V, D, and J gene