PET移植物的表面修饰概述.ppt

Development of thrombus-resistant and cell compatible crimped polyethylene terephthalate cardiovascular grafts using surface co-immobilized heparin and collagen 运用将肝素和胶原在PET织状移植物表面共固化的技术，以提高其抗血栓性能以及细胞相容性 Abstract Short-term patency of polyethylene terephthalate (PET) cardiovascular grafts is determined mainly by the inherent thrombogenicity and improper endothelialization following grafts implantation. The aim of the present study was to immobilize heparin to develop thrombus resistant grafts. Additionally, collagen was co-immobilized to enhance the host cell compatibility. woven and knitted forms of crimped PET grafts of grafts Surface modify produce functional carboxyl groups covalent immobilization of heparin or co-immobilization of heparin/collagen the end-point connect Characterized by FTIR XPS Blood coagulation test platelet deposition test host cell adhesion and growth test Fig. 1. SEM micrographs of unmodified woven (A) and knitted (B) forms of crimped PET grafts. The multifilament PET threads in woven grafts are fabricated in an over-and-under pattern, while PET threads in knitted grafts are looped. Methods 1.Surface modification of crimped PET grafts Briefly, slices (2 × 2 cm2) from woven and knitted forms of crimped PET grafts were cleaned in 20ml of 50% ethanol solution for 15min in an ultrasonic water bath (Sonorex RK 100H, Bandelin,Germany). Subsequently, the grafts were rinsed several times with distilled water under stirring and then oven dried at 55 °C for 2 h. The cleaned grafts were dipped in 20ml of 1% NaOH solution and incubated in a boiling water bath for 1 h. The modified grafts were rinsed with distilled water and oven dried at 55 °C for 2 h. The obtained grafts are abbreviated as PET-COOH. PET 20ml of 50% ethanol solution， 15min ultrasonic water bath distilled water stirring oven dried 55 °C, 2h cleaned grafts 20ml of 1% NaOH solution boiling water bath ，1h PET-COOH 2.Grafting of ethylenediamine onto PET-COOH Crimped PE