RGQ安装时应用培训概述.ppt

The Rotor-Gene Q 应用培训 旋转模式 光学通路 光学通路 PCR 转子和PCR管类型 1 PCR 转子和PCR管类型 2 加热器 HRM工作流程 扩增 设置正常的PCR步骤和HRM步骤，用嵌入式染料 (e.g SYTO 9) 每个基因型都应设置Control(eg.已确定的wild-type, heterozygote, variant样品) 检测扩增曲线和扩增效率 (扩增不好，HRM的结果也不好) HRM 分析 运行HRM 分析 指定controls 观测结果 HRM运行设置 HRM分析 HRM分析 仪器的维护保养 问题解决 Online Help文件： HelpContents(或在New Run对话框中点击“Help”) 出现Online Help文件。包含了RGQ软件和硬件的详细信息（例如，如何装载样品，设置实验，进行特殊的分析等），可以据关键词有哪些信誉好的足球投注网站。 * Encourage the use of Auto gain for all applications. Ensure customers understand the use of it and implications if it is set incorrectly. * Name lists can be inserted manually or copied and pasted from another file or used from a previous run. The type of the sample is important for analysis. Note – samples labelled as None will not appear in any analysis. Only samples labelled with Standard will be allowed concentrations Groups – can be assigned if statistical data may be required for a group of samples that are not replicates ie treated vs untreated of multiple biological replicates Given conc – can be in any format chosen from the drop down list above Selected – whether the sample is shown on the trace data Also show how the colours can be changed by highlighting. 38 samples per day 38 samples per day 38 samples per day * Quick start wizard: Any of the default profile scan be altered while setting up a run – the default are simply the most commonly used times and temperatures Please note the ‘perform last run’ feature uses the profile and setting from the run last OPEN on that computer not necessarily actually run. * Quick start wizard: Any of the default profile scan be altered while setting up a run – the default are simply the most commonly used times and temperatures Please note the ‘perform last run’ feature uses the profile and setting from the run last OPEN on that computer not necessarily actually run. * Quick start wizard: Any of the default profile scan be altered while setting up a run – the default are simply the most commonly used times and