Lecture_11_Chromatography_.pptx

Chromatography;Terms;Chromatography;Chromatographic Separation;Classifying of Chromatography;Liquid Solid Chromatography, LSC;Liquid Liquid Chromatography, LLC;GC Advantages;GC Disadvantage;LC Advantage;LC Limitation;Column Materials;Column Type;GC Stationary Phases;矽膠及其衍生物是管柱的主要材料，稱為silica gel based column ;Silica gel (矽膠); totally porous particle shape: spherical or irregular shape particle size: 3, 4, 5, 6, 7, 10, 20, 40 μm pore size: 50-4000 ? ;正相層析法(normal phase chromatography)polar stationary phasenon-polar mobile phase吸附層析法(adsorption chromatography); silica－Sil column DIOL－DIOL column NO2－nitro column CN－cyano or nitrile column N(CH3)2－dimethyl amino column NH2－amino column ;逆相層析法 (reverse phase chromatography) non-polar stationary phasepolar mobile phase 分配層析法(partition chromatography) ;藉由含較低極性的固定相與含較高極性(甚至含水)的移動相兩者極性之差異，分析物與可固定相進行分配，可用於非極性物質及極性物質的分析 ;Alkyl groupC1 (methyl)C4 (butyl)C8 (octyl)C18 (octa decyl silane，ODS);General Rule of Thumb for Retention Behavior;;Retention Mechanisms;Ion pair chromatography對偶離子層析 ;常用試劑tri-n-hexylaminetri-n-octylaminetetra ethyl ammonium hydroxidetetra-n-butyl ammonium hydroxide (TBA) ;鹼性分析物;sodium 1-hexanesulfonate CH3(CH2)5SO3Na為試劑, 流通C18管柱時 CH3(CH2)5SO3-與Si-C18作用成Si-C18 CH3(CH2)5SO3- RxN+ + Si-C18 CH3(CH2)5SO3- Si-C18 CH3(CH2)5SO3- RxN+ ;Analytical Application of LC;Constants;Machine;Generalized GC;GC Injection Systems;Split Flow;Volatile Analysis ;Flame Ionization Detector (FID);Electron Capture Detector (ECD);Diode-array Detector;Working Ranges for GC Detectors;Generalized LC;Generalized Diagram of LC ;UV Cutoff;Solvent Strength ;Effect of Solvent Strength ;Effect of Mobile Phase ;Operation;Operation;Column care;Elute Volume;Column linear velocity(線速度)(體積流速/截面積);溫度效應 ;Effect of Temperature Program;Determination of tea catechins;Chromatographic analysis of standard catechins and caffeine and the effect of column temperature on their s