Chapter4ToolsofHumanMolecularGenetics精品资料.ppt

Chapter 4 Tools of Human Molecular Genetics;;ANALYSIS OF INDIVIDUAL DNA AND RNA SEQUENCES（个体DNA和RNA序列的分析）;Molecular Cloning（分子克隆）Restriction Enzymes（限制酶）;The Language Of Genomics And Molecular Genetics （基因组学和分子遗传学的术语）;;;;;;;;;;;Vectors（载体）Recombinant DNA technology（重组DNA技术）;Plasmids（质粒）;;Libraries（文库）;Complementary DNA (cDNA) Libraries （cDNA文库）;;Screening Libraries with Nucleic Acid Hybridization Probes（用核酸杂交探针筛选文库）;;;Genome Database Resources（基因组数据库资源）;METHODS OF NUCLEIC ACID ANALYSIS（核酸分析方法）;Southern Blotting（DNA印迹法）;;Analysis with Allele-Specific Oligonucleotide Probes（等位基因特异的寡核苷酸探针分析）;Northern or RNA Blotting（RNA印迹法或Northern印迹法）;THE POLYMERASE CHAIN REACTION（聚合酶链式反应）; ;Quantitative PCR（定量PCR）;DNA SEQUENCE ANALYSIS（DNA序列分析）;ADVANCED TECHNIQUES USING DIGITAL IMAGE CAPTURE OF FLUORESCENCE-TAGGED NUCLEOTIDES（用数码图像捕捉荧光标记的核苷酸的先进技术）;Fluorescence In Situ Hybridization to Chromosomes（染色体荧光原位杂交）;;Comparative Genome Hybridization（比较基因组杂交）;;RNA Expression Arrays（RNA表达阵列）;Clinical Applications of Expression Arrays for Molecular Phenotyping and Functional Pathway Analysis（表达阵列在临床中的应用：分子表型分型和功能代谢通路分析）;;WESTERN BLOT ANALYSIS OF PROTEINS（蛋白质印迹分析）;;The Molecular Analysis of a Human Mutation（人类突变的分子分析）