参考资料--对天然多糖的抗氧化活性研究.docxVIP

学院代号 10716 学号110050801108专业代码 081302密级 公开陕 西 中 医 学 院Shaanxi University of Chinese Medicine学士学位论文对天然多糖的抗氧化活性研究学位申请人曹柯专业名称制药工程申请学位类型工学学士学位指导教师姓名王露老师论文提交日期2013年6月对天然多糖的抗氧化活性研究姓名：曹柯 指导老师：王露老师【摘要】：多糖是一大类广泛存在于动植物和微生物体内的生物高分子物质，许多天然多糖具有增强免疫、抗氧化、降血糖、抗病毒等生物活性，天然多糖还具有毒副作用小、疗效好等优点，现在多糖已成为天然药物及保健品研发中的重要组成部分。本文从实验原理、实验方法等方面对天然多糖抗氧化活性的常见评价方法进行综述，详细介绍了化学分析法中的自由基（ABTS自由基阳离子、DPPH自由基、超氧自由基、羟自由基）清除实验、脂质过氧化抑制实验等评价方法；描述了以生物细胞为模型的CAA抗氧评价方法；概述了在动物体内进行的抗氧化活性评价方法及指标，为相关领域中抗氧化天然多糖的研究和开发提供实验理论基础。【关键词】：天然多糖； 抗氧化活性； 评价方法；The antioxidant activity of natural polysaccharide researchName: Cao Ke Teacher:Wang Lu【 abstract】 :Polysaccharide is a broad categories in a wide variety of plants and microorganisms in vivo biological macromolecule material, many natural polysaccharides have enhanced immune, antioxidant, fall blood sugar, anti-viral biological activity, such as natural polysaccharide has poison The advantages of small side effects, good curative effect, polysaccharide has now become an important part of natural medicines and health products research and development.This article from the experiment principle and experiment method of natural polysaccharide antioxidant activity of the common evaluation methods were reviewed, detailed introduces the chemical analysis of free radicals (ABTS radical cation, DPPH free radical, superoxide free radical, hydroxyl free radicals) removal experiment, lipid peroxidation inhibition experiment evaluation methods, such as;Describes the biological cells as the model of CAA antioxidant evaluation method;Outlined in animals on antioxidant activity evaluation methods and indicators, for oxidation of natural polysaccharides in related areas to provide theoretical basis for research and development.【 key words 】: Natural polysaccharides；antioxidant activity；evaluation methodology 1.天然多糖的抗氧化活性许多天然多糖对各种活性氧( Reactive oxygen species，ROS )具有清除作用，能减少脂质过氧化产物丙二醛(MDA)的生成量，提高抗氧化酶活性(超氧化物歧化酶 SOD、谷胱甘肽过氧化物酶GSH—Px等)，表现出多种途经的抗氧化作用．多糖具有广泛的生物活性，而抗氧化作用是