Proteinexpressionofcardiomyocyteproteinkinases.ppt

Protein expression of cardiomyocyte protein kinases. Stephen J. Fuller et al. Cardiovasc Res 2015;108:87-98 Published on behalf of the European Society of Cardiology. All rights reserved. ? The Author 2015. For permissions please email: journals.permissions@. Protein expression of cardiomyocyte protein kinases. Protein kinases in NVMs A or AVMs B were affinity purified, then identified and quantified by mass spectrometry MS . Expression of each kinase was calculated as the percentage of the MS signal for that kinase relative to the total MS signal for all kinases detected. The area of each circle in the diagram is proportional to the percentage value of the kinase relative to the total pool of kinases detected. Data are shown for the principal MAPK cascades, other important cardiomyocyte kinases, components of the Akt pathway pink, although p70S6k and Gsk3 are also phosphorylated by ERK1/2 and RSKs, respectively , and other kinases within the 30 most highly represented kinases. Relatively under-investigated kinases in cardiomyocytes are highlighted in dark blue. Common names are in capitals; otherwise gene symbols are used.