中国马铃薯晚疫病菌AFLP遗传多样性分析.docVIP

中国马铃薯晚疫病菌AFLP遗传多样性分析 杨志辉 朱杰华( 张凤国 河北农业大学植物保护学院 河北省农作物病虫害生物防治工程技术研究中心 保定 071001 摘 要：应用AFLP分子标记检测了我国部分马铃薯主要产区马铃薯晚疫病菌的遗传多样性及不同地区菌株间的亲缘关系。在200对引物组合中，利用6个菌株筛选出12对多态性好、带型清晰的引物组合。利用这12对引物组合对1997-2002年间采自我国黑龙江、河北、四川和云南4省的50株菌株进行了PCR扩增，共扩增出922条谱带，其中多态性标记530条，占57.5。利用NTSYS pc软件中UPGMA算法构建了我国马铃薯晚疫病菌的亲缘关系树状图，聚类分析结果表明我国马铃薯晚疫病菌的遗传多样性与病原菌的地理来源有一定的相关性，而与交配型、生理小种和对甲霜灵的抗性无明显的相关性。用POPGENE软件计算了各群体间的遗传多样性参数，结果表明我国马铃薯晚疫病菌的遗传多样性程度不高，不同地区种群间分化不明显。 致病疫霉，分子标记，遗传变异，群体结构 Genetic diversity of Chinese isolates of Phytophthora infestans revealed by AFLP analysis Yang zhi-Hui zhu jie-Hua* ZHANG Feng-Guo Department of Plant Pathology, Agricultural University of Hebei, Research Centre of P1ant Pathogen and Pest Control of Hebei Province, Baoding 071001, China Abstract：The genetic diversity of the populations of Phytophthora infestans from some major potato production regions in China were detected by amplified restriction fragment polymorphism (AFLP) analysisAmong 200 combinations of primer pair screened, 12 combinations could generate consistent polymorphic bands using six tested isolates. The twelve combinations were used to amplify the genomic DNA of 50 isolates collected in China from 1997 to 2002. A total of 922 AFLP bands were obtained, and 530 of them，covering 57.5showed polymorphism. Cluster analysis using the unweighted pair-group method with arithmetic averages (UPGMA) separated 50 isolates into five AFLP groups which were correlated to groups defined by geographical origin, however, they were not correlated to groups defined by mating type, or response to metalaxyl and virulence. Parameters of genetic diversity calculated by POPGENE software indicated that the genetic diversity level of Phytophtora infestans population in China was not high. Key words: Phytophthora infestans, molecular marker, genetic variation, population structure 19世纪中叶马铃薯晚疫病菌Phytophthora infestans de Bary首次成功地从起源中心墨西哥随种薯经美国传入欧洲，而后传遍全球Goodwin et al. 1994）。由于晚疫病菌是异宗配合真菌，在Hohl Iselin1984）于瑞士发现A2交配型以前，除墨西哥