2-鱼类麻醉.ppt

* Huazhong Agricultural University ? 2009 Li Dapeng Fish Anesthesia 李大鹏 Li Dapeng Ph.D. 华中农业大学水产学院 College of Fishery, Huazhong Agricultural University Huazhong Agricultural University ? 2009 Li Dapeng Introduction ? Fish often encounter stress induced by transportation, handling, surgery, and so on ? Effective anesthesia can reduce physiological or psychological pain from stress Huazhong Agricultural University ? 2009 Li Dapeng ? 2009 Li Dapeng Anesthesia methods Huazhong Agricultural University MS222 Clove oil Chemical 2-phenoxyethanol Physical Others Electricity ( DC , AC) Low temperature Huazhong Agricultural University 化学性方法 1. 2. 3. 4. 5. MS-222（烷基磺酸盐间位基苯甲酸乙酯） 丁香油（clove oil） 对氨基苯甲酸乙酯（benzocaine） 2- 苯氧基乙醇（2-phenoxyethanol） CO 2：不能完全消除痛觉 5 ? 2009 Li Dapeng 6 MS-222 ? 通过FDA 批准可以直接用于 食用鱼 ? 麻醉效果良好，使用剂量： 50 —200 mg/L ? 麻醉效果与水温的相关性 很大 Huazhong Agricultural University ? 2009 Li Dapeng 7 a professional anesthetic 鱼 类 麻 醉 ? 2009 Li Dapeng 8 Clove oil 水温低于15℃，需要乙醇助溶 （1: 10） ? 2009 Li Dapeng 9 Huazhong Agricultural University ? 2009 Li Dapeng 10 MS-222 versus Clove oil ? 2009 Li Dapeng ? The plasma cortisol levels in anaesthetized fish suggest that anesthetics are effective as short-term stress reducers CT = control, MS= MS-222, CL= clove oil, C2 =CO2 ? Feed intake in fish treated with clove oil or MS-222 was lower than in the controls. ? There were no differences in feed intake among anaesthetised groups. ? Plasma cortisol concentrations were elevated 48 h after anaesthetizations—— unknown reasons ? 2009 Li Dapeng 13 手术检查及术后缝合 Huazhong Agricultural University ? 2009 Li Dapeng 14 ? Clove oil has been regarded as an effective and acceptable alternative to other anesthetics. ? Confinement without anesthesia produces a smaller stress response than with the anesthetics. Huazhong Agricultural University ? 2009 Li Dapeng 15 物理性方法 ? 低温 ? 瞬间致死 ? 电击（DC shock） ? 2009 Li Dapeng 16 － ＋ V A 电麻醉装置图