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在体短距离细小神经记录复合神经动作电位的最佳方法
在体短距离细小神经记录复合神经动作电位在体周围神经记录复合神经动作电位的研究很少,尤其在体短距离细小神经记录复合神经动作电位尚未有研究报道华山医院肌电图室多次探索进行预实验记录复合神经动作电位,不仅发现了记录的最佳方法,而且发现了小的不同电极之间的距离对复合神经动作电位参数有明显影响。实验在完全游离大鼠正中神经后,难以记录到复合神经动作电位,但保留神经中部系膜,将地线置于系膜下,两端放置记录和刺激电极,即可恒定记录到复合神经动作电位。研究结果表明:在大鼠正中神经记录复合神经动作电位,记录电极间距离和刺激电极间距离均以5mm为最佳,刺激电极至记录电极间最佳距离为10mm,顺向记录优于逆向记录。该方法不仅可以为基础研究提供参考临床探索短的臂丛神经根部或干部记录复合神经动作电位提供新研究思路。
记录复合神经动作电位
Article: The optimal distance between two electrode tips during recording of compound nerve action potentials in the rat median nerve by Yongping Li1, 2, Jie Lao1, Xin Zhao1, Dong Tian1, Yi Zhu1, Xiaochun Wei2 1 Department of Hand Surgery of HuaShan Hospital, Fudan University; Key Laboratory of Hand Reconstruction, Ministry of Healthy; Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, China; 2 Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
Li YP, Lao J, Zhao X, Tian D, Zhu Y, Wei XC. The optimal distance between two electrode tips during recording of compound nerve action potentials in the rat median nerve. Neural Regen Res. 2014;9 2 :171-178.
欲获更多资讯: Neural Regen Res
Optimal distance between two electrode tips during recording of compound nerve action potentials
The distance between the two electrode tips can greatly infuence the parameters used for recording compound nerve action potentials. To investigate the optimal parameters for these recordings in the rat median nerve, Prof. Jie Lao and colleagues from HuaShan Hospital, Fudan University in China dissociated the nerve using different methods and compound nerve action potentials were orthodromically or antidromically recorded with different electrode spacings. Compound nerve action potentials could be consistently recorded using a method in which the middle part of the median nerve was intact, with both ends dissociated from the surrounding fascia and a ground wire inserted into the muscle close to the intact part. A distance of 5 mm between recording and stimulating e
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