Illuminasequencingtechnology.ppt

* * * * * * Rice includes need to access content from related wild spp. Look how three different groups see the need for genome level sequencing to allow the impacts needed for future marker discovery that will give information content for breeding the superior plant lines needed for Food Feed to meet the 2050 global productivity goals. * Here’s a comment from a recent publication that gets straight to the point. [PAUSE TO ALLOW AUDIENCE TO READ IT]. We are learning about the read lengths required to efficiently and successfully perform assemblies of novel genomes. Read length barriers vary depending on the size and complexity of the genome. Based on Chaisson’s paper, we’ve illustrated here the approximate read length barriers for a bacterium [CLICK] and a simple eukaryote [CLICK]. The read-length barriers for mammalian genomes have not yet been defined, but we anticipate a read-length barrier in the range of 100-150 bases [CLICK]. The Genome Analyzer comfortably handles this range. * * * Key feature – 2 independent flowcells. These enable instrument scalability – run 1 or 2 for a 100G or 200G system, or 1B or 2B read system. This means that even lower output/read suited applications are well served by HiSeq 2000. The additional benefit is experimental flexibility- applications that require different read lengths can be run on each flowcell – an example is that a whole genome sequencing and de novo sequencing study (eg. Of human and microorganism) could be run on one flowcell at 2 x 100bp reads, and the other could be running ChIP-seq and gene expression samples at 1 x 50bp read length. Each flowcell is controlled independently; hence, a run can be started/stopped independent of the other. Key feature – convenient flowcell loading dock- easy to use, just place flowcells on dock and activate the vacuum switch. Position 1 applies the vacuum, indicator prompts you to switch to position 2 when connection and registration are established. * Simply drop bottles in