ClC-2论文ClC-2 非小细胞肺癌 半定RT-PCR Westernblot 免疫化学.doc

【关键词】ClC-2 非小细胞肺癌 半定RT-PCR Westernblot 免疫化学 【英文关键词】clc-2 non-small-cell lung cancer semi-quantitative RT-PCR Western blotimmunochemistry ClC-2论文：电压门控性氯离子通道ClC-2对非小细胞肺癌的作用及表达意义 【中文摘要】通过观察肺癌发生、侵袭及转移时氯通道ClC-2在人非小细胞肺癌中的表达情况，探讨ClC-2在非小细胞肺癌中的表达及临床意义方法：实验分为对照组(癌旁正常肺组织)和实验组(肺良性病组、肺鳞癌组和肺腺癌组),应用半定量RT-PCR及Westernblot，免疫组化等方法检测各组ClC-2mRNA、ClC-2蛋白表达水平及观察ClC-2在细胞及组织中的表达和分布差异。并对ClC-2在非小细胞肺癌中的表达情况进行初步分析。结果：每实验组中ClC-2mRNA和ClC-2蛋白均一定的表达。基因产物ClC-2mRNA表达水平上与对照组及肺良性病组相比，非小细胞肺癌组的ClC-2mRNA表达量明显增多，肺良性病组比对照组略表达增多。ClC-2蛋白表达量在非小细胞肺癌及肺良性病组均比对照组表达增多。通过免疫组织化学法也证实ClC-2在非小细胞肺癌中广泛、大量地分布。结论：三种实验方法均证实了ClC-2在非小细胞肺癌中过表达。提示ClC-2作为电压门控性性离子通道，通过各种机制在临床肺癌发生、侵袭及转移，肺部疾病发生过程中起重要作用。ClC-2可能成为临床治疗非小细胞肺癌的新分子靶点，深入研究ClC-2与肺癌发生、发展关系中提供新的思路。 【英文摘要】:To investigate the role of voltage-gated chloride channel clc-2on non-small-cell lung cancer and the significance of the expression of it, we examine the gene expression of chloride channel clc-2in human non-small-cell lung cancer during occurrence, invasion and metastasis.Materials and Methods:Samples involved in this research were divided into four groups. They are:①Pulmonary adenocarcinoma;②squamous lung carcinomas;③Pulmonary tuberculosis;④ormal lung tissue besides lung cancer. The groups of non-small-cell lung cancer and benign pulmonary diseases are the experimental groups. The group of normal lung tissue beside lung cancer is the control group. In each experiment, group①and group②comply with the same principles of gender, pathological T staging and the degree of differentiation. The rest groups were selected at random for the comparison and analysis within those groups. From the results of semi-quantitative RT-PCR, Western blot and immunohistochemistry analysis, the expressions of clc-2mRNA and clc-2protein was determined, and difference in the the expression and distribution of clc-2among different kinds of cells and tissues were also assessed. Furthermore, we preliminary analysed the expression of clc-2in non-small-cell lung cancer cells. The4