苗药痛风停汤对急性痛风性关节炎模型大鼠血清IL―8的影响.docVIP

苗药痛风停汤对急性痛风性关节炎模型大鼠血清IL―8的影响 　　【摘 要】目的：探讨苗药痛风停汤对急性痛风性关节炎模型大鼠白细胞介素-8的影响。方法：将48只雌性SD大鼠随机分为空白对照组、模型对照组、痛风停治疗组及双氯芬酸钠对照组，每组12只。复制急性痛风性关节炎大鼠模型，采用光镜观察模型大鼠关节病理变化，酶联免疫吸附（ELISA）法检测大鼠血清白细胞介素-8。结果：模型对照组、痛风停治疗组、双氯芬酸钠对照组白细胞介素-8的表达均高于空白对照组（P 0.01）；与模型对照组比较，痛风停治疗组、双氯芬酸钠对照组白细胞介素-8的水平较低（P 0.01）。结论：痛风停汤能够降低急性痛风性关节炎SD大鼠白细胞介素-8的表达，减轻关节滑膜炎症。 　　【关键词】 关节炎，急性痛风性；白细胞介素-8；苗药痛风停汤；大鼠 　　doi：10.3969/j.issn.2095-4174.2014.01.003 　　Effects of Miao Medicine Tongfengting Decoction on the Serum IL-8 in Model Rats 　　with Acute Gouty Arthritis 　　ZHONG Qin，LIU Zheng-qi，AN Yang，ZHANG Xiong-feng，MA Wu-kai，FU De-hong 　　【ABSTRACT】 Objective：To investigate the effects of Miao medicine Tongfengting Decoction on the serum IL-8 in model rats with acute gouty arthritis.Methods：48 female SD rats were randomly and equally divided into the blank control group，the model control group，the Tongfengting control group and the diclofenac sodium control group.Duplicated the rat models with acute gouty arthritis and observed the pathological changes of rat models with optical microscope. The enzyme linked immunosorbent assay （ELISA） was used to detect the serum IL-8 in model rats.Results：The expressions of IL-8 in the model control group，the Tongfengting group and the diclofenac sodium control group were higher than that of the blank control group （P 0.01）；compared with the model control group，the IL-8 level in the Tongfengting control group and the diclofenac sodium control group was lower （P 0.01）.Conclusion：Tongfengting Decoction can decrease the expression of IL-8 in SD rats with acute gouty arthritis，alleviating the inflammation of joint synovium. 　　【Keywords】 arthritis，acute gout；interleukin-8；Miao medicine Tongfengting Decoction；rats 　　痛风是嘌呤代谢紊乱和/或肾小管源性的尿酸排泄障碍所引起亦称I型结晶沉积病。尿酸盐晶体作用于关节周围组织、滑膜细胞，刺激这些细胞释放多种炎症介质，其中致炎因子白细胞介素-8（IL-8）在急性痛风性关节炎的发病中起重要作用。因此，降低炎症组织中的IL-8的水平从而减轻急性期症状成为治疗急性痛风的一个新视点。笔者前期的临床研究中显示，苗药痛风停汤能够缓解痛风性关节炎的临床症状[1-3]。本实验通过对比观察痛风停汤对急性痛风性关节炎SD模型大鼠膝关节滑膜中IL-8的表达水平及滑膜病理的变化，探讨痛风停汤治疗急性痛风性关节炎的作用机制。