增生性瘢痕演变过程中成纤维细胞内皮素.doc

增生性瘢痕演变过程中成纤维细胞内皮素-1的表达变化 向 军 王西樵 刘英开 青 春 陆树良 上海交通大学 医学院瑞金医院 【摘要】 目的:观察增生性瘢痕在发生和演变过程中，成纤维细胞中内皮素-1（ET-1）的表达。方法:取烧伤病人创面上皮化后不同时期增生性瘢痕的组织、正常皮肤组织标本，体外培养后，检测培养的成纤维细胞中ET-1 mRNA的表达情况；同时应用Masson’s染色观察瘢痕组织中胶原变化和免疫组化技术测定瘢痕组织中微血管CD34的表达。结果：在增生性瘢痕发生和演变过程中，成纤维细胞ET-1 mRNA的表达水平逐渐升高，于增生期达到高峰，消退期逐步下降，成熟期基本恢复正常。同时，瘢痕组织中CD34的阳性表达也呈现逐步升高，增生期达到高峰，而后逐步降低的变化。Masson’s染色显示：胶原密度和含量在早期瘢痕开始增加，增生期达到高峰，消退期胶原含量和密度降低，成熟期胶原含量基本恢复正常。结论：烧伤后增生性瘢痕发生和演变过程中，成纤维细胞ET-1表达水平呈现早期逐步升高，增生期达到高峰，消退期下降的变化，这些变化可能与瘢痕组织中胶原和微血管的病理改变密切相关。 【关键词】 成纤维细胞；内皮素-1；增生性瘢痕 【Abstract】 Objective The study is to investigate the rules of regulated expressions of endothelin-1(ET-1) by fibroblasts in the progress of human hypertrophic scar progression. Methods The human hypertrophic scars of different stages (early, proliferative, regressive and mature scars) from the burn patients were collected and processed for Massion’s staining, as well as CD34 detections by immunohistochemical staining.Meanwhile, the isolated fibroblasts from the samples were cultured and total RNA were extracted for ET-1mRNA detection. Results During the hypertrophic scar progression, experiments revealed that the expression of ET-1mRNA increased in the early scar, which reached a peak in the hypertrophic scar, and then decreased significantly in the regressive scar, while turned to normal level in the mature scar. The changes of CD34 expression were the same as ET-1.In the mean time, the change law of collagens contents and density observed by Massion’s staining was also likely to ET-1. Conclusion In the progress of human hypertrophic scar progression, the changes of ET-1 expressions by the fibroblasts were well correlated with the pathological changes of collagens and micro vessels in the scar tissues. 【Key words】 fibroblast；endothelin-1；hypertrophic scar 近年来，内源性生长因子与创面修复、瘢痕发生的关系愈来愈受到人们的关注。既往研究认为[1]，生长因子内皮素-1（ET-1）主要由血管内皮细胞和血管平滑肌细胞产生，其主要作用是促血管化；而目前的研究发现： 成纤维细胞也能产生ET-1，并在创面修复过程中与微血管生成和胶原分泌合成密切相关[2]。本文拟通过检测不同时期瘢痕组织中成纤维细胞ET-1的表达水平来评价瘢痕演变过程中ET-1变化和