多种水溶性及脂溶性维生素含量之测定.doc

多種水溶性及脂溶性維生素含量之測定-毛細管電泳及其他方法之評估 Determination of water-soluble and fat-soluble vitamins - by capillary electrophoresis and evaluate with other methods 研究生: 孫蓓菁 Sun Pei Ching 指導教授: 溫惠美 陳景川 [摘要]The purpose of the research is to clone and study express the genes encoding 2-acetyl-1-pyrroline biosynthetic enzymes glycerol kinase and dihydroxyacetone kinase in aromatic rice Oryza sativa cv. TN71 . The glycerol kinase GK and dihydroxyacetone kinase DAK gene of Oryza sativa TN71 had been cloned by using polymerase chain reaction PCR cloning technology. The degenerate primers were designed based on conserved amino acid sequences in the PCR for O- GK and O-DAK gene amplification. End-to-end PCR was performed to amplify full- length GK and DAK cDNA. Rice calli were treated with different levels of proline and glycerol to study the expression of both genes, which was analysed by real time PCR and calibrated with the housekeeping actin cDNA. The results showed that both GK and DAK expression in the calli were induced by glycerol in a dose dependent manner. Keywords: 2-acetyl-1-pyrroline aromatic ri