新城病磁珠化诊断试剂之研制.doc

豬流行性下痢病例報告 豬瘟研究組 鄧明中 副研究員 摘要 Case Report: Porcine Epidemic Diarrhea Ming-Chung Deng Abstract In January 2014, the suckling piglets exhibited severe diarrhea and death in pig farms in Pingtung and Yunlin County, Taiwan. The specimens were submitted to Animal Health Research Institute on January 21. It’s very difficult to differentiate transmissible gastroenteritis TGE , porcine epidemic diarrhea PED and porcine rotavirus infection by clinical syndromes. According to virus isolation and RT-PCR, the preliminary diagnosis was PED infection. The PED was confirmed by transmission electron microscope TEM examination and gene sequencing. Furthermore, the partial M gene of isolated PEDV was compared with other PEDV sequence in Genbank. The isolated PEDV was 100% identity with US strain isolated in 2013, and 98.1% identity with classical strain CV-777. Until now, the outbreak of PED has gradually slowed down. The positive rate of PEDV was 75.61% 31/41 , including 31 pig farms distributed in 9 counties.