生物制药工艺学题 第九章 亲和纯化技术.doc

第九章 亲和纯化技术 一、填空题 、亲和层析中非专一性吸附有、、。 、亲和过滤指的是将和结合运用，它包括和两大方法。 二、选择题 1、下面关于亲和层析载体的说法错误的是 （） A载体必须能充分功能化。 B载体必须有较好的理化稳定性和生物亲和性，尽量减少非专一性吸附。 C载体必须具有高度的水不溶性和亲水性。 D理想的亲和层析载体外观上应为大小均匀的刚性小球。 2、制备亲和柱时，应首先选用的配基是 （） A大分子的 B小分子的 C中等大小的 D不确定 、亲和层析的洗脱过程中，在流动相中减去配基的洗脱方法称作（ A. 阴性洗脱 B. 剧烈洗脱 C. 正洗脱 D. 负洗脱三、名词解释 1、（Affinity-based resersed micellar extraction）（secondary-effect affinity precipitation）亲和萃取： 6、负洗脱： 四、问答题 1、亲和层析的原理是什么？主要特点是什么？ 进行说明。设 KL=10-7，求[L0] （6分） 3、何谓“手臂”？其长短与亲和层析效果有何联系？为什么？ 4、从亲和沉淀的机理和分离操作的角度出发，简述亲和沉淀纯化技术的优点。 loan management (post) review: 1, approval criteria have been implemented; 2, the contract is correct; 3, in line with the contract, payment; 4, no other major cases against loan security;、Reviewed by: date of record form 9: qilu bank borrowing by single years of maximum amount a maximum no-loan borrowers: borrowers: ... Borrower name number, line of credit expiry date year month day to year month day maximum number of years in the loan contract are the top line opened, the borrowed and requested your bank opened credit lines above, is hereby requested. Borrower signature: date agreed to open credit lines. Customer Manager signature: date agreed to open credit lines. Loan review signature: date line cancellation-I did not use the loan amount, your bank credit lines to be cancelled. -Credit loan principal repayment, your bank credit lines to be cancelled. Borrower signature: date-unused credit lines, agree to write off loans. -Credit loan principal repayment, agree to write off loans. Customer Manager signature: date case, agree to write off loans. Loan review signature: date of record form 16: qilu self credit for credit card cancellation application form name of borrower ID number and credit limit expiration date year month day to year month day year contract number self-service card-I do not use credit, your bank credit lines to be cancelled. -Credit l