基金项目国家自然科学基金项目名称葛根素注射剂致溶血.docVIP

基金项目：国家自然科学基金 项目名称：葛根素注射剂致溶血反应机制与评价方法研究（NO 葛根素注射液对正常及糖尿病家兔溶血作用研究 曹烨君1，丁选胜2 （1.江苏省常州市第四人民医院临床药学室,江苏常州 213001；2.中国药科大学临床药学教研室,江苏南京 210009） 摘要 目的：研究葛根素注射液在正常和糖尿病家兔体内给药后的溶血规律，为临床安全合理用药提供依据。方法：采用四氧嘧啶复制1型糖尿病家兔模型，并与正常健康家兔进行对照，设置10、20、40mg体重三个剂量组，每周期给药10天，在给药前以及每个给药周期的第2、4、6、8、10天分别采血，观察红细胞形态并比较体外溶血差异。结果：正常家兔和糖尿病家兔红细胞在给药后的溶血率较之给药前均有升高，糖尿病组的溶血率高于正常组，在给药后6~8天溶血率升至最高。溶血率与给药剂量和给药时间成正相关。结论：临床使用葛根素注射液，给药时间应控制在8天以内，若需长时间用药，需要在每个用药周期后间隔4~5天的清洗期，以保证用药安全性，避免发生溶血反应。 关键词：葛根素注射液；糖尿病家兔；体内给药；溶血反应 Hemolytic Effect of Puerarin Injection on Normal and Diabetic Rabbits CAO Ye-Jun1, DING Xuan-Sheng2 （1.No.4 hospital of Changzhou, Changzhou, Jiangsu 213001 ; 2. Department of Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009） ABSTRACT：Objective：To study the rule of puerarin injection hemolysis on normal and diabetic rabbits,and provide evidence for clinical rational adminstration of drug. Methods: This study established type 1 diabetic rabbits model by alloxan, comparing to healthy rabbits. The dosage of treatment are 10, 20, 40mg/kg. The treatment of healthy and diabetic rabbits was divided into 2 periods. Every period had 10 days. Before and during the treatment collected blood. The study showed that after treatment, the hemolysis rate increased in healthy and diabetic rabbits.Results：The study showed that after treatment, the hemolysis rate increased in healthy and diabetic rabbits. In 6~8d, the hemolysis rate was highest. The hemolysis rate had a positive correlation with dosage and time. The hemolysis of diabetic rabbits was more serious than the healthy group. Conclusions：In clinical practice, the treatment should be controlled under 8 days. If the patients should have a long time of treatment, it must be divided into some periods, which has 4 to 5 days as an interval. That would ensure drug safety and avoid hemolytic reaction. Key Words: Puerarin injection; Diabetic rabbits; Pathological states in vivo; Hemolytic reaction. This work is supported