毕业论文--基于ndhf序列的植物遗传关系分析设计论文.doc

SHANDONGＵＮＩＶＥＲＳＩＴＹ　ＯＦ　ＴＥＣＨＮＯＬＯＧＹ 毕业论文 基于ndhF序列的植物遗传关系分析 学 院： 生命科学学院 专 业： 生物技术 中文摘要 随着分子生物学和生物信息学的快速发展, 基于各种分子数据的分析已成为植物系统学研究的主要手段, 其中以DNA序列的比较分析应用最为广泛。植物拥有3套基因组, 按进化速率由快到慢依次为核基因组( nDNA) 、叶绿体基因组( cpDNA) 和线粒体基因组(mtDNA) (Wolfe et al. , 1987) 。每套基因组内不同区域的核苷酸进化速率不同, 为各分类阶元的系统学研究提供了丰富的变异来源。 ndhF是叶绿体功能基因，该基因共含有21个编辑位点, 且这21个位点均为部分编辑。生物信息学分析及与其它物种比对结果表明, ndhF C290位编辑可能会影响该蛋白的正确折叠。进一步使用单克隆酶切方法测定了不同胁迫处理对ndhF C290位编辑效率的影响。 论文基于ndhF序列对几种植物进行了遗传关系分析。首先从GenBank数据库中筛选植物序列，所得序列利用软件进行相关分析，根据遗传距离及分子系统学分析对ndhF片段长度对比，研究并分析菊属、紫草属、狗尾草属植物的遗传关系。 关键字：ndhF，分子系统学，遗传关系分析，GenBank数据库Abstract With the development of molecular biology and bioinformatics of rapid development, based on a variety of molecular data analysis has become the main means of plant systematics research, of which DNA comparative sequence analysis is most widely used. The plant has 3 sets of genome, according to the evolutionary rate from fast to slow in the nuclear genome, chloroplast genome ( nDNA ) ( cpDNA ) and mitochondrial genome ( mtDNA ) ( Wolfe et al.,1987). Each genome within different regions of nucleotide evolution rate, for each taxon of the system science research provides a rich source of variation. ndhF is a function of chloroplast gene, the gene contains 21 edit sites, and these 21loci are part of editing. Bioinformatics analysis and other species than the results show that, ndhF C290editors may influence the protein is correctly folded. Further use of monoclonal enzyme digestion method for the determination of the different stress treatments on ndhF C290editing efficiency Based on the ndhF sequence of several plant performed a genetic relationship analysis. First, from the GenBank database screening plant sequences, the sequence using the software according to the correlation analysis, genetic distance and molecular phylogenetic analysis of ndhF fragment length comparison, research and analysis of genetic relationship of orchids Key words: ndhF genetic, molecular syst