js南京出国留学中介构.doc

为了纪念科学引文索引（SCI）诞生五十周年，Nature委托汤森路透列出了数据库中的百大高引论文。荣登榜首的那篇文章你一定猜不到。 据介绍，这份百强名单的入围资格是引用次数超过12119。令人意外的是，许多特别知名的论文并未入榜，比如DNA双螺旋结构的确定。而1951年 发表的一种蛋白定量方法，狂揽三十多万次引用，成为了最大的赢家。该文章的作者，美国生化学家Oliver Lowry也对此感到困惑。 ? Rank Title Times cited 1 Protein measurement with the folin phenol reagent. 305148 2 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. 213005 3 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. 155530 4 DNA sequencing with chain-terminating inhibitors. 65335 5 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. 60397 6 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. 53349 7 Development of the Colle-Salvetti correlation-energy formula into a functional of the electron density. 46702 8 Density-functional thermochemistry. III. The role of exact exchange. 46145 9 A simple method for the isolation and purification of total lipides from animal tissues. 45131 10 Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. 40289 11 Nonparametric estimation from incomplete observations. 38600 12 Basic local alignment search tool. 38380 13 A short history of SHELX. 37978 14 Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. 36410 15 A revised medium for rapid growth and bio assays with tobacco tissue cultures. 36132 16 Generalized gradient approximation made simple. 35405 17 Mini-mental state: A practical method for grading cognitive state of patients for clinician. 34532 18 A rapid method of total lipid extraction and purification. 32131 19 Detection of specific sequences among DNA fragments separated by gel-electrophoresis. 31904 20 The neighbor-joining method: A new method for reconstruct